Local delivery to biomaterials scaffold

局部递送至生物材料支架

基本信息

批准号：
17500312
负责人：
TACHIBANA Akira
金额：
$ 1.6万
依托单位：
Osaka City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17500312/
关键词：
local delivery FGF2 BMP2 chitin binding domain cellulose binding domain バイオマテリアル Chitin binding domain Cellulose binding domain

项目摘要

The seats of chitin and cellulose were constructed by casting of chitin and cellulose solution in dimethylactamide containing LiCl. The expression vector plasmids for expression of fusion proteins, chitin binding domain-FGF2, cellulose binding domain-FGF2 and FGF2, were constructed by the new method, sequential SmaI cloning. The fusion proteins were expressed in Escherichia coli and purified by the glutathione affinity column chromatography. The Chitin-binding domain-FGF2 protein was specifically bound on chitin biomaterials, that was detected by immunological techniques using anti-FGF2 antibody. The Chitin-binding domain-FGF2 protein was also bound on acetylated area of chitosan biomaterials. The cellulose-binding domain-FGF2 protein was specifically bound on cellulose biomaterials, that was detected by immunological techniques using anti-FGF2 antibody. On the cellulose biomaterial scaffold bound cellulose binding domain-FGF2, osteoblastic cells MC3T3-E1 were grown without serum. The fusion protein, chitin binding domain-BMP2 also expressed osteoblastic differentiation activity to osteoblastic cells MC3T3-E1 on the chitin biomaterials, chitin seat and acetylated chitosan plate. The fusion proteins, chitin/ cellulose binding domain-FGF2/ BMP2, were locally delivered to specific biomaterials.

通过在含有二甲基二甲酰胺的二甲基二酰胺中铸造几丁质和纤维素溶液来构建几丁质和纤维素的座椅。用于融合蛋白，几丁质结合结构域-FGF2，纤维素结合结构域-FGF2和FGF2的表达载体质粒是通过新方法的顺序SMAI克隆构建的。融合蛋白在大肠杆菌中表达，并通过谷胱甘肽亲和色谱柱色谱法纯化。几丁质结合结构域-FGF2蛋白特别结合了几丁质生物材料，该材料是通过使用抗FGF2抗体的免疫技术检测到的。几丁质结合结构域-FGF2蛋白也与壳聚糖生物材料的乙酰化区域结合。纤维素结合结构域-FGF2蛋白特别结合了纤维素生物材料，该纤维素生物材料是通过使用抗FGF2抗体的免疫技术检测到的。在纤维素生物材料支架结合结合结构域-FGF2上，成骨细胞MC3T3-E1无血清生长。融合蛋白，几丁质结合结构域-BMP2还表达成骨细胞分化活性与成骨细胞MC3T3-E1在几壳质材料，几架蛋白座和乙酰化壳聚糖板上。融合蛋白，几丁质/纤维素结合结构域-FGF2/ BMP2，局部递送到特定的生物材料中。