Controlled release of growth factors for bone regeneration

控制释放生长因子以促进骨再生

• 批准号: 23500543
• 负责人: TACHIBANA Akira
• 金额: $ 3.33万
• 依托单位: Osaka City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23500543/
• 关键词: 徐放システム; FGF2; キチン; キチンゲル; キチン結合ドメイン; リゾチーム応答性徐放; chitin binding domain; BMP2/7; キトサン; セルロース; セルロース結合ドメイン; pH感受性

Chitin gel was obtained by the acetylation of chitosan in solution. Basic growth factors did not bind to non treated chitin gel, whereas tightly bound to carboxymethylated chitin gel. Chitin binding domain-FGF-2 was bound to chitin gel and not released by PBS washing. Chitin binding domain-FGF-2 was released by degradation of chitin gel with lysozyme. During degradation of chitin gel with lysozyme, chitin fragment was released at a burst, but chitin binding domain-FGF-2 was not. Most of chitin binding domain-FGF-2 that was released from gel were re-bound to the gel, only low amount of FGF-2 was released out. Therefore, ideal controlled release without a burst release was realized.

甲壳素凝胶是由壳聚糖在溶液中乙酰化而成。基本生长因子不与未经处理的甲壳素凝胶结合，而与羧甲基化的甲壳素凝胶紧密结合。几丁质结合结构域--成纤维细胞生长因子-2结合在几丁质凝胶上，PBS洗涤后不释放。用溶菌酶降解几丁质凝胶，释放出几丁质结合结构域--成纤维细胞生长因子-2。在溶菌酶降解甲壳素凝胶的过程中，甲壳素片断突然释放，而几丁质结合结构域-成纤维细胞生长因子-2不释放。凝胶中释放的甲壳素结合区--成纤维细胞生长因子-2大部分被重新结合到凝胶上，只有少量的成纤维细胞生长因子-2被释放出来。从而实现了理想的无突释的控释。

项目成果

キチンゲルを用いたFGF2の徐放

使用甲壳素凝胶持续释放 FGF2

• 发表时间: 2012
• 作者: 安間 大;立花 亮;田辺 利住
• 通讯作者: 田辺 利住

キチンゲルを用いたFGF2の徐放/Controlled release of FGF2 using chitin gel

使用甲壳素凝胶控制 FGF2 的释放

• 发表时间: 2012
• 作者: 安間大;立花亮;田辺利住
• 通讯作者: 田辺利住

キチンゲルを用いたFGF2の徐放/Controlled release of FGF2 using chitin gel バイオインターフェース先端マテリアルの創生

使用甲壳素凝胶控制释放 FGF2 创建先进的生物界面材料

• 发表时间: 2013
• 作者: 安間大;立花亮;田辺利住
• 通讯作者: 田辺利住