Vaslcular Dilatation and Improvement of Tissue Perfusion Induced by Low-Frequency Ultrasound: Influence of Nitric Oxide in Tissue

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Background : Noninvasive application of low-frequency ultrasound causes arterial vasodilation and improves tissue perfusion. Although the mechanism is unclear, a nitric oxide (NO)-dependent mechanism is considered. We evaluated whether low-frequency ultrasound can produce expression of endothelial nitric oxide synthase (eNOS) in the rat heart.Methods : Using 22 anesthetized Sprague-Dawley rats, 45 kHz ultrasound (750 mW) was insonated to the hearts (group A: 30 minutes of insonation × 1 day; group B: 30 minutes of insonation × 6 days). The expression of eNOS was analyzed by Western blotting.Results : Compared to the expression of eNOS in sham group (100%), that in group A did not change (107±24%); however, that in group B increased (141±38%).Conclusion : The finding of the current study indicates that the energy of low-frequency ultrasound can produce expression of eNOS.