Monoamine dynamics in control of spontaneous physical activity in rats

单胺动力学控制大鼠自发体力活动

• 批准号: 17500429
• 负责人: NAKAYA Yutaka
• 金额: $ 2.18万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17500429/
• 关键词: exercise; monoamine oxidase; norepinephrine; micro-dialysis; serotonin; orexin; atrial thrombosis; adhesion molecules; ノルエピナフリン; 運動; 海馬; モノアミン; 高血圧; 血栓; 交感神経

We generated an original Wistar line of rats that displayed increased levels of wheel running, SPORTS (Spontaneously-Running-Tokushima-Shikoku). Male SPORTS rats ran voluntarily in a running wheel almost six times longer than male control Wistar rats. We examined the running activity of SPORTS rat, to clarify neurological mechanisms for wheel running by rodents, especially its relation with the hippocampal norepinephrine (NE) system including the levels of NE, adrenergic receptors, and degradation enzymes for monoamines. In the hippocampus of SPORTS rats, the level of NE in extracellular fluid was elavated, whereas the level in the homogenate of the whole tissue was decreased. Local administration of a2-adrenergic receptor antagonist yohimbine, but not of a2-agonist clonidine, into the hippocampus suppressed high running activity in SPORTS rats. The protein expression and the activity levels of monoamine oxidase A (MAOA), a critical enzyme for the degradation of NE, were decreased in the hippocampus of SPORTS rats, which in turn increased extracellular NE level. Thus, inhibition of MAOA activity in normal Wistar rats markedly increased wheel-running activity. These results indicate that decreased MAOA activity, elevation of extracellular NE, and a2-adrenergic receptors in the hippocampus determine the neural basis of the psychological regulation of exercise behavior in SPORTS rats.We also found that SPORTS rats frequently are associated with left atrial thrombi, presumably due to increased coagulability, increased expression of adhesion molecules, and endothelial damage due to hypertension. Thus, this rats also serve as a model of atrial thrombosis in hypertension.

我们产生了一个原始的Wistar系大鼠，显示出增加水平的车轮运行，运动（自发运行-德岛四国）。雄性运动大鼠自愿运行在一个运行轮几乎六倍于雄性对照Wistar大鼠。本研究通过观察大鼠运动后的活动，探讨啮齿类动物转轮运动的神经机制，特别是与海马去甲肾上腺素（NE）系统的关系，包括NE、肾上腺素能受体和单胺降解酶的水平。在运动大鼠海马，细胞外液中NE含量升高，而整个组织匀浆中NE含量降低。局部给药α 2-肾上腺素受体拮抗剂育亨宾，而不是α 2-激动剂可乐定，到海马抑制高运动大鼠的活动。运动后大鼠海马内NE降解的关键酶单胺氧化酶A（MAOA）的蛋白表达和活性水平降低，细胞外NE水平升高。因此，在正常Wistar大鼠中抑制MAOA活性显著增加跑轮活动。这些结果表明，MAOA活性降低，细胞外NE和海马α 2-肾上腺素能受体的升高决定了运动大鼠运动行为的心理调节的神经基础。我们还发现，运动大鼠经常与左心房血栓，可能是由于增加凝血，粘附分子的表达增加，和内皮损伤，由于高血压。因此，该大鼠也可用作高血压心房血栓形成的模型。

项目成果

Ginsenoside Re, a main phytosterol of Panax ginseng, activates cardiac potassium channels via a nongenomic pathway of sex hormones

• DOI: 10.1124/mol.106.028134
• 发表时间: 2006-12-01
• 期刊: MOLECULAR PHARMACOLOGY
• 影响因子: 3.6
• 作者: Furukawa, Tetsushi;Bai, Chang-Xi;Kurokawa, Junko
• 通讯作者: Kurokawa, Junko

Human airway trypsin-like protease stimulates human bronchial fibroblast proliferation in a protease-activated receptor-2-dependent pathway

• DOI: 10.1152/ajplung.00098.2005
• 发表时间: 2006-02-01
• 期刊: AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
• 影响因子: 4.9
• 作者: Matsushima, R;Takahashi, A;Yasuoka, S
• 通讯作者: Yasuoka, S

A pore-forming toxin produced by Aeromonas sobria activates cAMP-dependent C1(-) secretory pathways to cause diarrhea.

温和气单胞菌产生的一种成孔毒素会激活 cAMP 依赖性 C1(-) 分泌途径，从而引起腹泻。

• 发表时间: 2005
• 期刊: FEMS Microbiol Lett 242
• 作者: Tanoue N;Takahashi A;Okamoto K;Fujii Y;Taketani Y;Harada N;Nakano M;Nakaya Y
• 通讯作者: Nakaya Y

Molecular cloning of a murine glycerol-3-phosphate acyltransferase-like protein 1 (xGPAT1)

• DOI: 10.1007/s11010-006-9321-5
• 发表时间: 2007-03-01
• 期刊: MOLECULAR AND CELLULAR BIOCHEMISTRY
• 影响因子: 4.3
• 作者: Harada, Nagakatsu;Hara, Sayuri;Nakaya, Yutaka
• 通讯作者: Nakaya, Yutaka

Angiotensin II activates intermediate-conductance Ca2+ -activated K+ channels in arterial smooth muscle cells.

• DOI: 10.1016/j.yjmcc.2006.07.010
• 发表时间: 2006-12
• 期刊: Journal of molecular and cellular cardiology
• 影响因子: 5
• 作者: Y. Hayabuchi;Y. Nakaya;Sonoko Yasui;K. Mawatari;K. Mori;Mitsujiro Suzuki;S. Kagami