Combined estrogenic effect of soybean food containing phytoestrogens and endocrine disruptor

含有植物雌激素和内分泌干扰物的大豆食品的联合雌激素作用

• 批准号: 17500546
• 负责人: KAWAZOE Sadahiro
• 金额: $ 1.02万
• 依托单位: Kyoto Prefectural University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17500546/
• 关键词: phytoestrogens; soybean food; endocrine disruptor; estrogenic effect; combined estrogenic effect

Isoflavones, such as genistein and daidzein, are phytoestrogens present in soybean foods and have estrogenic activity. In this study, we examined the combined estrogenic effect of soybean extract (Nijiru) containing human intake dose level of isoflavones (10 mg/kg/day) as soybean food and endocrine disruptor.Soybeans were boiled for 40 min at 120"C in water, and Nijiru was produced and was then freeze-dried to make a powder. Nijiru powder contained daidzin, glycitin, genistin, daidzein, glycitein, and genistein. Nijiru (1900-6600 mg/kg corresponding to dose of total isoflavone 10 mg/kg) was administered to ovariectomized ICR mice by oral gavage for 11 consecutive days. The synthetic endocrine-active hormone diethylstilbestrol (DES) (0.05 mg/kg) was then co-administered orally with Nijiru and continued for another 3 days. The relative uterine weight (% of body weight) of Nijiru+DES treatment was slightly higher than that of DES alone. Similarly, synthetic endocrine-active compound bisphenol A (BPA) (300 mg/kg) or pesticide methoxychlor (MX) (50 mg/kg) were co-administered orally with Nijiru and continued for another 3 days. Although Nijiru+MX treatment increased (vs. MX alone) the relative uterine weight, Nijiru+BPA treatment did not increase (vs. BPA alone) the relative uterine weight.

异黄酮，如染料木黄酮和大豆黄酮，是大豆食品中存在的植物雌激素，具有雌激素活性。在这项研究中，我们检查了含有人类摄入剂量水平的异黄酮（10 mg/kg/天）的大豆提取物（Nijiru）作为大豆食品和内分泌干扰物的综合雌激素作用。将大豆在120°C水中煮沸40分钟，产生Nijiru，然后冷冻干燥制成粉末。Nijiru粉末含有大豆苷， 黄豆黄素、染料木黄酮、大豆黄酮、黄豆黄素和染料木黄酮。 Nijiru（1900-6600 mg/kg，相当于总异黄酮剂量10 mg/kg）通过口服管饲法连续11天给予切除卵巢的ICR小鼠。合成内分泌活性激素己烯雌酚 (DES) (0.05 mg/kg) 然后与 Nijiru 共同口服给药，并持续另外 3 天。 Nijiru+DES 治疗的相对子宫重量（占体重的%）略高于单独 DES 的情况。同样，合成内分泌活性化合物双酚 A (BPA)（300 毫克/千克）或农药甲氧氯 (MX)（50 毫克/千克） 与 Nijiru 联合口服给药，并持续 3 天。虽然Nijiru+MX治疗增加了（相对于单独的MX）相对子宫重量，但Nijiru+BPA治疗没有增加（相对于单独的BPA）相对子宫重量。