Testing of Enterococci as Novel Fecal Contamination Indicator in Public Water Body

公共水体中肠球菌作为新型粪便污染指标的测试

• 批准号: 17510026
• 负责人: IWAHORI Keisuke
• 金额: $ 1.79万
• 依托单位: University of Shizuoka
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17510026/
• 关键词: Public water body; Enterococci; Enterolert method; M-enterococcus method; Johkasou system; Gray water; Fecal contamination indicator; 大腸菌; 大腸菌群; 糞便汚染; Colilert法

This research aimed at the applicability of Enterococci as a novel fecal contamination indicator in the public water body. The obtained results can be summarized as follows;Comparing with various enterococci enumeration methods in both pure and mixed cultures, using 7 type strains of Escherichia coli, coliform group, Enterococci and their pseudopositive bacteria, it was obvious that both Enterolert and M-enterococcus methods were available, the former was recommended as the Standard Methods for the Examination of Water and the latter was as that of Wasewater in Japan. In confirmed test, the numbers of E.coli, coliform group and Enterococci were shown to be correlative and both methods had a high relation in the samples of small-scale domestic wastewater treatment system (Gappei-shori Johkasou).Nextly, the fecal contamination indicators of the water samples in the Tomoe Basin and the effluents in the Johkasou systems installed at this Basin were surveyed. As the results, Enterococci enumerated by both Enterolert and M-enterococcus methods were applicable as the fecal indicator in the public water body. The ratio of the Tandoku-shori Johkasou to the Johkasou systems in this Basin was investigated at the end of FY 2005, because Enterococci was detected in the gray water. This ratio was more than 90% and the possibility was shown that the gray water including the fecal indicators ran down into the Tomoe Basin. Therefore, the conversion of the Tandoku to the Gappei-shori Johkasou was suggested to create the secure and safe Tomoe Basin.Consequently, it was shown that Enterococci with E.coli and coliform group was effective as a novel fecal contamination indicator in the public water body.

本研究旨在探讨肠球菌作为一种新的粪便污染指标在公共水体中的适用性。结果表明：用大肠埃希菌、大肠菌群、肠球菌及其假阳性菌7种典型菌株，对纯培养和混合培养的肠球菌计数方法进行比较，表明Enterolert法和M-肠球菌法均适用，前者被推荐为《水质检验标准方法》，后者被推荐为日本《废水检验标准方法》。在确认试验中，小型生活污水处理系统（Gappei shori Johkasou）的水样中大肠杆菌、大肠菌群和肠球菌的数量具有相关性，两种方法的相关性较高。结果表明，肠球菌计数法和M-肠球菌计数法均可作为公共水体粪便指示菌。由于在灰水中检测到肠球菌，因此在2005财政年度末调查了该流域Tandoku-shori Johkasou与Johkasou系统的比例。这一比例超过90%，表明包括粪便指示剂在内的灰水可能流入Tomoe盆地。因此，为了建设安全的友江流域，建议将丹渡改为Gappei shori Johkasou。结果显示，大肠杆菌和大肠菌群的肠球菌作为公共水体中粪便污染的新指标是有效的。