Development of separation processes using ionic liquids

1)在本研究中，我们考察了室温离子液体(RTILs)作为膜溶液能否用于分离石油中的烃类，以及利用支撑液膜分离发酵液中的有机酸和青霉素G。我们用1-正丁基-1-正己基和1-正辛基-3-甲基咪唑的六氟磷酸盐和季铵盐作为离子液体。用离子液体浸渍聚偏氟乙烯多孔膜，制得支撑液膜。有机硫、氮化合物、青霉素G和乳酸均成功地通过离子液体膜转运。(2)对映体化合物在医药和农业中具有重要意义，因为每种对映体对人体的活性不同。特别是手性二醇类化合物是一种性能优良的中间体，因此需要采用手性二元醇的制备方法。离子液体是一种新型的酶促反应介质，具有不挥发性、易燃性、热稳定性和高极性等特点。在酶促反应中表现出较高的稳定性和对映体选择性。本研究利用具有对映体选择性的环氧化物水解酶在缓冲液/离子液体两相体系中对环氧乙烷进行动力学拆分，制备对映体二醇。利用粘红酵母产生的环氧化物水解酶对1，2-环氧己烷具有较高的对映体选择性进行了研究。粘红酵母ATCC201718产生的环氧化物水解酶不需要从全细胞中提取，具有很高的催化活性，并且对R-对映体具有良好的对映体选择性。

1) In this study, we examine whether room-temperature ionic liquids (RTILs) as a membrane solution can be utilized for hydrocarbon separation from petroleum, and organic acid and penicillin G from the fermented broth by using supported liquid membrane. We used the hexafluorophosphates of 1-n-butyl-, 1-n-hexyl-and 1-n-octyl-3-methyl imidazolium and quaternary ammonium salts as an ionic liquid. The supported liquid membrane was prepared by impregnating the porous polyvinylidene fluoride film with the ionic liquid. Organic sulfur and nitrogen compounds, peniciliin G and lactic acid were successfully transported through the membrane based on the ionic liquids.(2) Enantiopure chemicals are important in pharmaceutical and agricultural, because each enantiomer shows different activity for human body. Specially, chiral diols are one of the most excellent compounds as intermediate materials, therefore enantiopure preparation method is required. Ionic liquid is a novel medium for enzymatic reactions and has characteristics such as nonvolatile, inflame ability, thermal stability and high polarity. It has shown high stability and enantioselectivity in enzymatic reaction. In this research, it was performed kinetic resolution of rac-epoxides by epoxide hydrolase, which has enantioselectivity, for preparing enantiopure diol in biphasic system of buffer/ionic liquid. Epoxide hydrolase from Rhodotorula glutinis, which had reported for high enantioselectivity for 1,2-epoxyhexane as substrate, was used for this research. Epoxide hydrolase from Rhodotorula glutinis ATCC201718 shows high catalytic activity without being extracted from whole-cell, and excellent enantioselectivity for R-enantiomer was confirmed.