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Development of novel extractive fermentation process of lactic acid with solvent-tolerant bacteria

耐溶剂细菌乳酸提取发酵新工艺的开发

基本信息

批准号：
13650833
负责人：
MATSUMOTO Michiaki
金额：
$ 2.3万
依托单位：
Doshisha University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13650833/
关键词：
Lactic acid fermentation Solvent tolerant bacteria Hiochii bacteria Solvent extraction Synergism Trioctylamine Ionic liquids Gram-positive bacteria

项目摘要

Lactic acid is mainly used in the traditional food industry as an additive, and has recently drawn attention as the raw material for the production of biodegradable polylactic acid. With an increase in demand for environmentally friendly materials, lactic acid production by fermentation methods has gained interest. The economics of the process, however, depend on the development of an effective recovery method for lactic acid from the broth, because the separation and purification steps account for up to 50% of the production costs. Solvent extraction has been proposed as a promising recovery technique as an alternative to the conventional precipitation process. Recently, in situ extractive fermentation processes, in which lactic acid is simultaneously recovered by solvent extraction during the production of lactic acid in the fermentation broth, have been deemed attractive from the perspectives of suppression of the end-product (lactic acid) inhibition and enhanced reactor efficiency. In the present study, in order to repress the toxicity of the extractant and organic solvent we developed the new process for in situ extractive fermentation by using Lactobacillus fruclivorans NRIC0224 (hiochibacterium), which was alcoholophilic and could grow in the presence of 1-deanol. Tri-n-octylamine (TOA) in decanol gave the highest extractability of lactic acid. It was found that yeast extract and peptone in fermentation broth inhibited to extract lactic acid. The toxicity of TOA to Lb.fructivorans NRIC0224 was tested. In the presence of TOA and calcium carbonate (CaCO_3), the bacterium could grow, consume glucose, and produce lactic acid.Based on these results, we examined the applicability of a column packed with alginate capsules containing the cells and calcium carbonate to the extractive fermentation of lactic acid. The extractive fermentation of lactic acid was successful from the points of view of cell growth and reduction of the end-product inhibition.

乳酸主要用作传统食品工业中的添加剂，最近作为生产可生物降解聚乳酸的原料引起了人们的关注。随着对环保材料的需求增加，通过发酵方法生产乳酸引起了人们的兴趣。然而，该工艺的经济性取决于从肉汤中回收乳酸的有效方法的开发，因为分离和纯化步骤占生产成本高达 50%。溶剂萃取已被认为是一种有前景的回收技术，可以替代传统的沉淀工艺。最近，从抑制最终产物（乳酸）抑制和提高反应器效率的角度来看，原位提取发酵工艺被认为是有吸引力的，其中在发酵液中生产乳酸的过程中通过溶剂萃取同时回收乳酸。在本研究中，为了抑制提取剂和有机溶剂的毒性，我们开发了利用嗜酒精乳杆菌NRIC0224（hiochibacteris）进行原位提取发酵的新工艺，该细菌具有嗜酒精性并且可以在1-脱醇存在下生长。癸醇中的三正辛胺 (TOA) 具有最高的乳酸萃取率。结果发现，发酵液中的酵母提取物和蛋白胨抑制乳酸的提取。测试了TOA对食果乳杆菌NRIC0224的毒性。在 TOA 和碳酸钙 (CaCO_3) 存在下，细菌可以生长、消耗葡萄糖并产生乳酸。基于这些结果，我们检查了装有细胞和碳酸钙的藻酸盐胶囊的柱在乳酸提取发酵中的适用性。从细胞生长和减少最终产物抑制的角度来看，乳酸的提取发酵是成功的。