Evolutional analysis of multicellularization in relation to STAT signals by use of Dictyostelium.

1.通过遗传筛选，分离到12个新的STATA抑制基因。DUTA基因的一段是STATA的抑制因子。我们证明了内源性DUTA通过酪氨酸磷酸化来负向调节STATA的活性，以防止顶峰。过表达的duA片段以负性方式抑制内源duA促进顶端生长。在被抑制的克隆中，部分活性STATA的酪氨酸磷酸化水平增加(Shimada和Kawata，2007)。我们正在鉴定另外两个抑制基因，NK20和sunB.4。我们分离了被认为与细胞程序性死亡有关的基因，并由STATA调节它们的表达。我们分离到一个零突变体，并正在对其进行鉴定。

1. We isolated 12 novel STATa suppressor genes by genetical screening.2. A segment of dutA gene was a suppressor of STATa. We proved endogenous dutA is negatively regulating STATa activity through tyrosine phosphorylation to prevent culmination. Overexpressed dutA segment was acting dominant negative fashion to inhibit endogenous dutA to promote culmination. There was an increased tyrosine phosphrylation of partially active STATa in the suppressed clones (Shimada and Kawata, 2007).3. We are characterizing two more suppressor genes, NK20 and sunB.4. We isolated genes supposed to be involved in programmed cell death and are regulated their expressions by STATa. We isolated one of null mutant and are characterizing it.