Evolutional analysis of multicellularization in relation to STAT signals by use of Dictyostelium.

使用盘基网柄菌对与 STAT 信号相关的多细胞化进行进化分析。

• 批准号: 17570190
• 负责人: KAWATA Takefumi
• 金额: $ 0.77万
• 依托单位: Toho University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17570190/
• 关键词: genes; signal transduction; evolution; development ＆ differentiation

1. We isolated 12 novel STATa suppressor genes by genetical screening.2. A segment of dutA gene was a suppressor of STATa. We proved endogenous dutA is negatively regulating STATa activity through tyrosine phosphorylation to prevent culmination. Overexpressed dutA segment was acting dominant negative fashion to inhibit endogenous dutA to promote culmination. There was an increased tyrosine phosphrylation of partially active STATa in the suppressed clones (Shimada and Kawata, 2007).3. We are characterizing two more suppressor genes, NK20 and sunB.4. We isolated genes supposed to be involved in programmed cell death and are regulated their expressions by STATa. We isolated one of null mutant and are characterizing it.

1.通过遗传学筛选，我们分离到12个新的STATa抑制基因. dutA基因的一个片段是STATa的抑制基因。我们证明内源性dutA通过酪氨酸磷酸化负调节STATa活性，以防止顶点。过表达的dutA片段以显性负性方式抑制内源dutA促进植株的生长。在抑制的克隆中，部分活性STATa的酪氨酸磷酸化增加（Shimada和Kawata，2007）。我们正在表征另外两个抑制基因，NK20和sunB。我们分离了被认为参与程序性细胞死亡的基因，并通过STATa调节其表达。我们分离了一个无效突变体，并对其进行了鉴定。

项目成果

Analysis of a homologue of the adducin head gene which is a potential target for the Dictyostelium STAT protein Db-STATa.

内收蛋白头基因同源物的分析，该基因是盘基网柄菌 STAT 蛋白 Db-STATa 的潜在靶标。

• 发表时间: 2006
• 期刊: International Journal of Developmental Biology 50(6)
• 作者: Aoshima;R;Hiraoka;R;Shimada;N;Kawata,T
• 通讯作者: Kawata,T

Analysis of a gene that is a potential target for the Dictyostelium STAT protein Dd-STATa and that encodes a protein homologous to the adducin head domain.

分析盘基网柄菌 STAT 蛋白 Dd-STATa 的潜在靶标基因，该基因编码与内收蛋白头结构域同源的蛋白。

• 发表时间: 2006
• 期刊: International Journal of Developmental Biology (未定)(印刷中)
• 作者: Aoshima;R.;Hiraoka;R.;Shimada;N.;Kawata;T.
• 通讯作者: T.

Evidence that non-coding RNA dutA is a multicopy suppressor of Dictyostelium STAT protein,Db-STATa.

有证据表明非编码 RNA dutA 是盘基网柄菌 STAT 蛋白 Db-STATa 的多拷贝抑制因子。

• 发表时间: 2007
• 期刊: Eukarytic Cell 未定(印刷中)
• 作者: Shimada;N;Kawata;T.
• 通讯作者: T.

Analysis of a homologue of the adducin head gene which is a potential target for the Dictyostelium STAT protein Dd-STATa.

内收蛋白头基因同源物的分析，该基因是盘基网柄菌 STAT 蛋白 Dd-STATa 的潜在靶标。

• 发表时间: 2006
• 期刊: International Journal of Developmental Biology 50(6)
• 作者: Aoshima;R.;Hiraoka;R.;Shimada;N.;Kawata;T.
• 通讯作者: T.

Evidence that non-coding RNA dutA is a multicopy suppressor of Dictyo stelium STAT protein, Dd-STATa.

有证据表明非编码 RNA dutA 是 Dictyo stelium STAT 蛋白 Dd-STATa 的多拷贝抑制因子。

• 发表时间: 2007
• 期刊: Eukaryotic Cell 6
• 作者: Shimada N. and Kawata;T.
• 通讯作者: T.