Functional analyses of cellulase genes in multicellular microbe and application to a new technology

多细胞微生物纤维素酶基因的功能分析及新技术应用

• 批准号: 21510230
• 负责人: KAWATA Takefumi
• 金额: $ 2.58万
• 依托单位: Toho University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21510230/
• 关键词: セルラーゼ; 細胞壁; 形態形成; バイオマス; 転写因子; 細胞性粘菌

Cellulose is a major component of the cell wall for cellular slime mould, Dictyostelium discoideum. It posses a cellulose synthetase gene and its function is important for normal morphogenesis. In this project, we focused on the cellulase genes catalyzing cellulose hydrolysis, cloned several of them and investigated the effects on morphogenesis as well as cellulose hydrolyzing activity. We showed inactivation of cellobiohydrolase(cbhA) gene, one of cellulase, by gene disruption affected morphogenesis. In addition, multiple Dictyostelium cellulases might act synergistically, possibly as a cellulosome, resulted in drastic increase of cellulose hydrolyzing activity. This implies Dictyostelium is a useful organism to produce bioethanol from cellulose.

纤维素是细胞黏菌盘基网柄菌细胞壁的主要成分。它具有纤维素合成酶基因，其功能对正常的形态发生很重要。在本项目中，我们重点研究了纤维素酶催化纤维素酶的基因，克隆了几个纤维素酶基因，并研究了它们对纤维素酶的形态发生和纤维素酶活性的影响。我们发现纤维素酶中的纤维二酸水解酶(CbhA)基因的失活会影响细胞的形态发生。此外，多种纤维素酶可能协同作用，可能是作为一个纤维素体，导致纤维素酶活性的急剧增加。这表明网柄网柄菌是一种利用纤维素生产生物乙醇的有用生物。

项目成果

SunB, a novel SUN domain-containing protein required for development of Dictyostelium discoideum

SunB，盘基网柄菌发育所需的一种新型含 SUN 结构域的蛋白质

• 发表时间: 2010
• 期刊: Development Growth and Differentiation
• 影响因子: 2.5
• 作者: Nao Shimada;Kei Inouye;Satoshi Sawai;Takefumi Kawata
• 通讯作者: Takefumi Kawata

Evidence for a functional link between Dd-STATa and Dd-PIAS, a Dictyostelium PIAS homologue

Dd-STATa 和 Dd-PIAS（盘基网柄菌 PIAS 同源物）之间功能联系的证据

• DOI: 10.1111/j.1440-169x.2011.01296.x
• 发表时间: 2011
• 期刊: Develop. Growth & Differ
• 作者: Kawata;T.;Hirano;T.;Ogasawara;S.;Aoshima;R. and Yachi;A.
• 通讯作者: A.

Identification of two tyrosine kinase-like (TKL) proteins that redundantly activate STATc in response to hyper-osmotic stress

鉴定两种酪氨酸激酶样 (TKL) 蛋白，它们在高渗透压应激时过度激活 STATc

• 发表时间: 2011
• 作者: Hai;L.V.;Araki;T.;Na;J.;Kawata;T.;Williams;J.G.;Eichinger;L.
• 通讯作者: L.

TKL tyrosine kinase phosphorylates and activates a non-metazoan STAT

TKL 酪氨酸激酶磷酸化并激活非后生动物 STAT

• 发表时间: 2011
• 作者: Araki;T.;Kawata;T. and Williams;J. G. A
• 通讯作者: J. G. A

Identification of two tyrosine kinase-like(TKL) proteins that redundantly activate STATc in response to hyper-osmotic stress

鉴定两种酪氨酸激酶样 (TKL) 蛋白，它们在高渗应激下过度激活 STATc

• 发表时间: 2011
• 作者: Hai;L. V.;Araki;T.;Na;J.;Kawata;T.;Williams;J. G. and Eichinger;L.
• 通讯作者: L.