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Generation of stress-resistant transgenic tomato containing a large amount of ascorbic acid

含有大量抗坏血酸的抗逆转基因番茄的产生

基本信息

批准号：
17580079
负责人：
ESAKA Muneharu
金额：
$ 2.3万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17580079/
关键词：
ascorbic acid acerola transgenic plant stress-resistant plant tomato vitamin C cDNAクローニング

项目摘要

Man is unable to synthesize ascorbic acid (vitamin C) and so depends on external sources such as plants for its supply. L-ascorbic acid attracts so much attention because of its important role in plants and relevance to man. It acts as an enzyme cofactor and an antioxidant and is involved in many different essential processes within the plant cell, including hormone and cell wall biosynthesis, stress resistance, photo-protection and cell growth. It has been established that GDP-D-mannose is involved in plant ascorbic acid biosynthesis. GDP-mannose is used for the biosynthesis of ascorbic acid, glycoprotein, L-fucose and cell wall polysaccharide. Any change in the expression of GDP-mannose pyrophosphorylase catalyzing the formation of GDP-mannose may affect the biosynthesis of ascorbic acid. The L-galactose which is released, through yet unclear steps, is oxidized to L-galactono-1,4-lactone and later converted to L-ascorbic acid by L-galactono-1,4-lactone dehydrogenase.I have investigat … More ed biosynthesis pathway and biological function of tomato plant. L-galactono-1,4-lactone dehydrogenase and GDP-mannose pyrophosphorylase are the vital enzymes in the proposed biosynthetic pathway for ascorbic acid in plants. The cDNAs encoding L-galactono-1,4-lactone dehydrogenase and GDP-mannose pyrophosphorylase have been cloned and sequenced from tomato. Northern hybridization analysis indicated that their relative mRNA levels correlated with the changing ascorbic acid content of tomato tissues, suggesting that L-galactono-1,4-lactone dehydrogenase and GDP-mannose pyrophosphorylase are involved in ascorbic acid biosynthesis.Acerola (Malpighia glabra L.) is one of the richest known natural sources of ascorbic acid (vitamin C). Thus, the expression of ascorbic acid-biosynthesizing enzymes is markedly high. Thus, I challenged to generate transgenic tomato containing a large amount of ascorbic acid in which acerola genes for ascorbic acid-biosynthesizing enzymes are introduced and over-expressed. Finally, the possibility of the generation of transgenic tomato that has resistance to environmental stresses by increasing their ascorbic acid content has been discussed. Less

人类无法合成抗坏血酸(维生素C)，因此依赖于植物等外部来源提供。L-抗坏血酸因其在植物中的重要作用和与人类的相关性而备受关注。它作为一种酶辅助因子和抗氧化剂，参与植物细胞内许多不同的基本过程，包括激素和细胞壁的生物合成、抗逆性、光保护和细胞生长。已证实GDP-D-甘露糖参与植物抗坏血酸的生物合成。甘露糖用于生物合成抗坏血酸、糖蛋白、L岩藻糖和细胞壁多糖。催化GDP-甘露糖形成的GDP-甘露糖焦磷酸化酶表达的任何变化都可能影响抗坏血酸的生物合成。释放出来的L-半乳糖通过尚不清楚的步骤被氧化成L-半乳糖基-1，4-内酯，然后被L-半乳糖基-1，4-内酯脱氢酶转化为L-抗坏血酸。我在…做了调查番茄的生物合成途径和生物学功能的研究进展。L-半乳糖基-1，4-内酯脱氢酶和甘露糖焦磷酸化酶是植物体内抗坏血酸生物合成途径中的两个重要酶。从番茄中克隆了编码L-半乳糖基-1，4-内酯脱氢酶和甘露糖焦磷酸化酶的基因。Northern杂交分析表明，它们的相对表达水平与番茄组织中抗坏血酸含量的变化相关，提示L-半乳糖基-1，4-内酯脱氢酶和甘露糖焦磷酸化酶参与了抗坏血酸的生物合成。是已知的抗坏血酸(维生素C)最丰富的天然来源之一。因此，抗坏血酸生物合成酶的表达明显较高。因此，我挑战培育含有大量抗坏血酸的转基因番茄，在其中引入并过度表达抗坏血酸生物合成酶的针叶蛋白基因。最后，讨论了通过提高抗坏血酸含量获得抗环境胁迫转基因番茄的可能性。较少

项目成果

期刊论文数量（31）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Expression of VvmybA1 Gene and Anthocyanin Accumulation in Various Grape Organs

DOI：
10.5344/ajev.2006.57.4.507
发表时间：
2006-12
期刊：
American Journal of Enology and Viticulture
影响因子：
1.9
作者：
S. Jeong;N. Goto-Yamamoto;K. Hashizume;S. Kobayashi;M. Esaka
通讯作者：
S. Jeong;N. Goto-Yamamoto;K. Hashizume;S. Kobayashi;M. Esaka

Quantification Methods of Genetically Modified Maize Contents Using Genomic DNAs Pretreted By Sonicaiton and Restriction Endonuclease Digestion For a Capillary-Type Real-Time PCR System with a Plasmid Reference Standard.

使用超声波预处理和限制性内切酶消化的基因组 DNA 进行转基因玉米含量的定量方法，用于带有质粒参考标准的毛细管型实时 PCR 系统。

DOI：
发表时间：
2006
期刊：
Biosci. Biotechnol. Biochem. 70(12)
影响因子：
0
作者：
Toyota A;Akiyama H;Sugimura M;Watanabe T;Sakata K;Siramasa Y;Kitta K;Hino A;Esaka M;Maitani T;Rapid
通讯作者：
Rapid

Rapid quantification methods of genetically modified maize contents using genomic DNAs pretreted by sonicaiton and restriction endonuclease digestion for a capillary-type real-time PCR system with a plasmid reference standard.

使用经超声和限制性内切核酸酶消化预处理的基因组 DNA 进行转基因玉米含量的快速定量方法，用于带有质粒参考标准的毛细管型实时 PCR 系统。

DOI：
发表时间：
2006
期刊：
Biosci. Biotechnol. Biochem. 70・12
影响因子：
0
作者：
Yamasaki;T.;Deguchi;M.;Fujimoto;T.;Masumura;T.;Uno;T.;Kanamaru;K.;Yamagata;H.;Mori T.et al.;Nishimura Y.et al.;Aoyama H.et al.;Toyota A.
通讯作者：
Toyota A.

Suppressed expression of the apoplastic ascorbate oxidase gene increases salt tolerance in tobacco and Arabidopsis plants.

DOI：
10.1093/jxb/eri167
发表时间：
2005-07
期刊：
Journal of experimental botany
影响因子：
6.9
作者：
A. Yamamoto;Md Nazmul H Bhuiyan;Rungaroon Waditee;Yoshito Tanaka;M. Esaka;K. Ôba;A. Jagendorf;T. Tak
通讯作者：
A. Yamamoto;Md Nazmul H Bhuiyan;Rungaroon Waditee;Yoshito Tanaka;M. Esaka;K. Ôba;A. Jagendorf;T. Tak

Rapid quantification methods of genetically modified maize contents using genomic DNAs pretreted by sonicaiton and restriction plasmid reference standard.

使用超声波预处理的基因组 DNA 和限制性质粒参考标准快速定量转基因玉米含量的方法。