Clarification of molecular mechanism for active biosynthesis and abundant accumulation of ascorbic acid in acerola.

阐明针叶樱桃抗坏血酸活性生物合成和大量积累的分子机制。

• 批准号: 21580113
• 负责人: ESAKA Muneharu
• 金额: $ 3.16万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580113/
• 关键词: アスコルビン酸; アセロラ; 遺伝子組換え; 遺伝子発現; 酸化ストレス; ストレス抵抗性植物; 熱帯性植物; ビタミンC; デヒドロアスコルビン酸; モノデヒドロアスコルビン酸; プロモーター; マンノースピロホスホリラーゼ

Acerola(Malpighia glabra) is an exotic fruit cultivated primarily for its abundant ascorbate content, which is over 20-fold higher than ascorbate content in lemon. The molecular mechanisms that regulate the biosynthesis and accumulation of a large amount of ascorbic acid in acerola have been clarified in this study. Also, ascorbic acid biosynthesis-related genes from acerola have been manipulated to generate ascorbic acid-rich transgenic plants. The overexpression of acerola ascorbate biosynthetic enzyme genes resulted in two-to three-fold increase in the ascorbate content of the transgenic plants.

刺梨是一种外来水果，栽培主要是因为它含有丰富的抗坏血酸，比柠檬中的抗坏血酸含量高出20多倍。本研究阐明了调控针叶中大量抗坏血酸生物合成和积累的分子机制。此外，来自针叶树的抗坏血酸生物合成相关基因已经被操纵来产生富含抗坏血酸的转基因植物。针叶抗坏血酸生物合成酶基因的过表达导致转基因植株的抗坏血酸含量增加了两到三倍。

项目成果

Identification of Potential Gene Targets for the Improvement of Ascorbate Contents of Genetically Modified Plants.

鉴定提高转基因植物抗坏血酸含量的潜在基因靶标。

• 发表时间: 2010
• 作者: Badejo AA;Esaka M.
• 通讯作者: Esaka M.

Overexpression of the acerola (Malpighia glabra) monodehydroascorbate reductase gene in transgenic tobacco plants results in increased ascorbate levels and enhanced tolerance to salt stress

• DOI: 10.1016/j.sajb.2011.08.005
• 发表时间: 2012-01-01
• 期刊: SOUTH AFRICAN JOURNAL OF BOTANY
• 影响因子: 3.1
• 作者: Eltelib, H. A.;Fujikawa, Y.;Esaka, M.
• 通讯作者: Esaka, M.

Gene expression of ascorbic acid biosynthesis related enzymes of Smirnoff-Wheeler pathway in acerola (Malpighia glabra)

针叶虎 (Malpighia glabra) 中 Smirnoff-Wheeler 途径抗坏血酸生物合成相关酶的基因表达

• 发表时间: 2009
• 期刊: J.Plant Physiol. 166
• 作者: Badejo A.A;Fujikawa Y;Esaka M.
• 通讯作者: Esaka M.

Increase in Ascorbate Content of Transgenic Tobacco Plants Overexpressing the Acerola (Malpighia glabra) Phosphomannomutase Gene

• DOI: 10.1093/pcp/pcn206
• 发表时间: 2009-02-01
• 期刊: PLANT AND CELL PHYSIOLOGY
• 影响因子: 4.9
• 作者: Badejo, Adebanjo A.;Eltelib, Hani A.;Esaka, Muneharu
• 通讯作者: Esaka, Muneharu

Characterization of secretory phospholipase A2 with phospholipase A1activity in tobacco

烟草中分泌型磷脂酶 A2 和磷脂酶 A1 活性的表征

• 发表时间: 2012
• 期刊: Nicotiana tabacum(L.). Lipids
• 作者: Fujikawa Y.;Fujikawa R.;Iijima N.;Esaka M.
• 通讯作者: Esaka M.