The study of the formation of extracellular matrix during the embryonic development

研究了鸡腺垂体胚胎发育过程中胶原结构的特点。首先用细胞浸渍法和扫描电镜对大鼠、猪和鸡腺垂体的三维胶原结构进行了比较，明确了胶原结构在这些动物物种之间的差异。利用免疫组织化学和扫描电镜技术对鸡腺垂体胶原结构的胚胎发育进行了研究。早在胚胎第8天（e8）就在组织中检测到I型胶原免疫反应，并在随后的阶段也观察到。然而，没有实质细胞被抗I型胶原和抗前胶原血清免疫染色。e8可见免疫阳性胶原隔由周围延伸至腺体，有小分支。胶原间隔在此阶段表现相对简单。在后期，隔膜逐渐分支形成更小的室室，形成细胞簇。扫描电镜观察，e8腺垂体可见较粗的胶原纤维丛。胶原网壁未完成，膜状结构和索状结构混杂。胶原网的密度和面积随着发育逐渐增大。在e20中，腺垂体的胶原结构与成年鸡相似。在组织切片上，鸡胚实质细胞对抗前胶原血清未见免疫阳性反应。另一方面，分散培养的鸡腺垂体细胞中有许多前胶原免疫反应细胞。部分细胞对胞体及周围底物抗I型胶原血清有免疫反应。这些结果提示鸡腺垂体实质细胞具有产生胶原蛋白的潜力。少

The characteristics of collagen architecture in the chicken adenohypophysis during embryonic development were investigated. At first, the three-dimensional collagen architecture of the adenohypophysis was compared among rat, pig and chicken using cell maceration method and scanning electron microscope and it was clarified that the of the collagen architecture was different among these animal species.Next, the embryonic development of collagen architecture in chicken adenohypophysis was investigated using immunohistochemical and scanning electron microscopic techniques. Type I collagen immunoreaction was detected in the tissue as early as in embryonic day 8 (e8) and also observed in following stages. However, no parenchymal cells were immunostained with the anti-Type I collagen and anti-pro-collagen sera. In e8, immunopositive collagen septa were observed as elongations from the circumference into the gland with a little branch. Collagen septa were relatively simple in the appearance in … More this stage. In later stages, the septa gradually branched to make smaller compartments to make the cell clusters. In the SEM observation, relatively coarse plexus of collagen fibrils were observed in e8 adenohypophysis. The wall of collagen mesh has not completed and membrane-like constructions and cord-like structures were intermingled. The density and area of collagen meshwork gradually increased with the development. In e20,the collagen architecture in the adenohypophysis was similar to that seen in the adult chicken.In the tissue section, there were no immunopositive reactions to the anti-procollagen serum in the parenchymal cells of the chicken embryo. On the other hand, many procollagen immunoreactive cells were observed in the dispersed adenohypophyseal cell culture from chick. Moreover, some cells had immunoreactions for anti-collagen type I serum in the cell body and the surrounding substrate. These results suggest that the parenchymal cells in chicken adenohypophysis have a potential to produce the collagen. Less