The study of the formation of extracellular matrix during the embryonic development

胚胎发育过程中细胞外基质形成的研究

基本信息

批准号：
17580257
负责人：
NISHIMURA Shotaro
金额：
$ 1.22万
依托单位：
KYUSHU UNIVERCITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17580257/
关键词：
Collagen Chicken embryo Ade nohypophysis Scanning electron microscopy Immunohistochemistry

项目摘要

The characteristics of collagen architecture in the chicken adenohypophysis during embryonic development were investigated. At first, the three-dimensional collagen architecture of the adenohypophysis was compared among rat, pig and chicken using cell maceration method and scanning electron microscope and it was clarified that the of the collagen architecture was different among these animal species.Next, the embryonic development of collagen architecture in chicken adenohypophysis was investigated using immunohistochemical and scanning electron microscopic techniques. Type I collagen immunoreaction was detected in the tissue as early as in embryonic day 8 (e8) and also observed in following stages. However, no parenchymal cells were immunostained with the anti-Type I collagen and anti-pro-collagen sera. In e8, immunopositive collagen septa were observed as elongations from the circumference into the gland with a little branch. Collagen septa were relatively simple in the appearance in … More this stage. In later stages, the septa gradually branched to make smaller compartments to make the cell clusters. In the SEM observation, relatively coarse plexus of collagen fibrils were observed in e8 adenohypophysis. The wall of collagen mesh has not completed and membrane-like constructions and cord-like structures were intermingled. The density and area of collagen meshwork gradually increased with the development. In e20,the collagen architecture in the adenohypophysis was similar to that seen in the adult chicken.In the tissue section, there were no immunopositive reactions to the anti-procollagen serum in the parenchymal cells of the chicken embryo. On the other hand, many procollagen immunoreactive cells were observed in the dispersed adenohypophyseal cell culture from chick. Moreover, some cells had immunoreactions for anti-collagen type I serum in the cell body and the surrounding substrate. These results suggest that the parenchymal cells in chicken adenohypophysis have a potential to produce the collagen. Less

研究了在胚胎发育过程中鸡腺嗜酸疾病中胶原蛋白结构的特征。首先，使用细胞浸润法和扫描电子显微镜在鼠，猪和鸡肉中比较了腺旋转分析的三维胶原蛋白结构，并阐明了这些动物物种之间的胶原蛋白结构不同。使用免疫组织和scanemicsic Inquicic进行了鸡蛋磷酸化的胶原蛋白结构的胚胎发展。 I型I胶原蛋白免疫反应早在胚胎第8天（E8）就在组织中检测到，并且在以下阶段也观察到。然而，没有用抗型I胶原蛋白和抗Pro-Collagen血清免疫染色体细胞。在E8中，观察到免疫阳性的胶原蛋白隔sa是从圆周到腺的伸长，并带有一个小分支。在此阶段，胶原蛋白隔板的出现相对简单。在后来的阶段，隔sa逐渐分支以制造较小的隔间以制造细胞簇。在SEM观察结果中，在E8腺旋转分析中观察到了相对粗糙的胶原纤维丛。胶原蛋白网的壁尚未完成，膜状结构和绳索状结构混合在一起。胶原蛋白网格工作的密度和面积随发展而逐渐增加。在e20中，腺旋hopophysy中的胶原蛋白结构与成年鸡肉相似。在组织截面中，对鸡胚胎副细胞中抗溢流膜血清没有免疫阳性反应。另一方面，在鸡肉分散的腺嗜碱性细胞培养物中观察到许多胶原蛋白免疫反应性细胞。此外，某些细胞对细胞体和周围底物的I型抗凝集型I型血清具有免疫反应。这些结果表明，鸡腺苷磷酸化中的副细胞具有产生胶原蛋白的潜力。较少的