INTERACTION AMONG SEVERAL TYPES OF LUTEAL CELLS DURING SPONTANEOUS AND INDUCED LUTEOLYSIS

自发和诱导黄体溶解过程中几种类型黄体细胞之间的相互作用

• 批准号: 17580246
• 负责人: ACOSTA T.j.
• 金额: $ 2.3万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17580246/
• 关键词: Bovine; Reproduction; Estrous cycle; Corpus luteum; Luteal endothelial cells; Nitric oxide; Prostaglandin F2 alpha; Cytokines; 繁殖; サイトカイン

1) Effects of storage and passage of bovine luteal endothelial cells on endothelin-1 and prostaglandin F2a production: Basal and tumor necrosis factor (TNF) a-stimulated production of endothelin-1 (ET-1) and prostaglandin (PG) F2a in unfrozen and frozen-thawed LEC until passage 10 was investigated. LEC were isolated from the corpus luteum using enzymatic digestion and magnetic beads coated with lectin BS-1. Basal production of PGF2a by LEC was not altered by passage and storage at-80°C, whereas basal ET-1 production decreased from passage 2 and 3 to passage 4 by unfrozen LEC and from passage 2 to passage 3 by frozen/thawed LEC. Exposure of LEC to TNFa increased ET-1 and PGF2a productionby unfrozen and frozen-thawed LEC from all passages examined.2) Acute changes in circulating concentrations of oxygen, nitric oxide and progesterone duringprostaglandin F2a-induced luteolysis were detected in coincidence with the decrease of circulating concentrations of progesterone (P4) in cows. The real-time changes in circulating concentrations of P4, nitrite and nitrate (stable metabolites of NO), and partial pressure of oxygen (p02) during PGF2a-induced luteolysis in cows. Blood samples were collected from the ovarian vein (OV), jugular vein (JV) and aorta abdominalis (AA) on Day 10 (ovulation = Day 0). PGF2a injection induced a significant decrease in the concentration of P4 in OV plasma within 2 h. The decrease in P4 concentrations was preceded by an increase in NO concentrations in OV, JV and AA. Basal p02 was significantly higher in OVP than in JV (mean 4.1 Kpa vs 6.4 Kpa). PGF2a injection increased p02 in OV between 0.25 and 4 h. The high basal p02 in OV and the acute increase in p02 and NO after PGF2a injection in ovarian circulation suggest that oxygen species are involved in decreasing P4 concentration during functional luteolysis.

1)牛黄体内皮细胞的储存和传代对内皮素-1和前列腺素F2 a产生的影响：研究了基础和肿瘤坏死因子（TNF）α刺激的未冷冻和冷冻解冻LEC中内皮素-1（ET-1）和前列腺素（PG）F2 a的产生，直至传代10代。采用酶消化和凝集素BS-1包被的磁珠从黄体中分离LEC。LEC的基础PGF_（2a）生成量不受传代和-80 ℃保存的影响，而未冻融LEC从第2代和第3代到第4代以及冻融LEC从第2代到第3代的基础ET-1生成量下降。（2）前列腺素F2 a诱导的黄体溶解过程中，循环氧、一氧化氮和孕酮浓度的急性变化与孕酮（P4）浓度的降低相一致。PGF 2a诱导的奶牛黄体溶解过程中P4、亚硝酸盐和硝酸盐（NO的稳定代谢物）循环浓度以及氧分压（p02）的实时变化。在第10天（排卵=第0天）从卵巢静脉（OV）、颈静脉（JV）和腹主动脉（AA）采集血样。注射PGF 2a后2 h内，OV血浆中P4浓度显著降低。P4浓度的下降之前，在OV，JV和AA的NO浓度的增加。OVP组的基础p02显著高于JV组（平均4.1 Kpa vs 6.4 Kpa）。PGF 2a注射在0.25和4 h之间增加OV中的p02。OV中的高基础p02和在卵巢循环中注射PGF 2a后p02和NO的急性增加表明氧物种参与功能性黄体溶解期间P4浓度的降低。

项目成果

Tool for reproductive management : New observation using color ultrasound scanning that were not possible with imaging only in black and white

生殖管理工具：使用彩色超声扫描进行的新观察，这是仅黑白成像无法实现的

• 发表时间: 2006
• 期刊: J.Reprod.Dev 52
• 作者: Bah MM;Acosta TJ;Pilawski W;Deptula K;Okuda K;Skarzynski DJ;Tatsukawa Y;Miyamoto A
• 通讯作者: Miyamoto A

Acute effects of prostaglandin F2a on systemic oxytocin and progesterone concentrations during the mid-or late-luteal phase in mares.

前列腺素 F2a 对母马黄体期中期或晚期全身催产素和黄体酮浓度的急性影响。

• 发表时间: 2007
• 期刊: Animal Reproduction Science. 97
• 作者: Utt MD;Acosta TJ;Wiltbank MC;Ginther 0J
• 通讯作者: Ginther 0J

Role of intraluteal prostaglandin F2α, progesterone and oxytocin in basal and pulsatile progesterone release from developing bovine corpus luteum

• DOI: 10.1016/j.prostaglandins.2006.01.002
• 发表时间: 2006-05-01
• 期刊: PROSTAGLANDINS & OTHER LIPID MEDIATORS
• 影响因子: 2.9
• 作者: Bah, Mamadou M.;Acosta, Tomas J.;Skarzynski, Dariusz J.
• 通讯作者: Skarzynski, Dariusz J.

Effects of storage and passage of bovine luteal endothelial cells on endothelin-1 and prostaglandin F2α production

• DOI: 10.1262/jrd.18142
• 发表时间: 2007-06-01
• 期刊: JOURNAL OF REPRODUCTION AND DEVELOPMENT
• 影响因子: 1.8
• 作者: Acosta, Tomas J.;Yoshioka, Shin;Okuda, Kiyoshi
• 通讯作者: Okuda, Kiyoshi

Effect of storage and passage of bovine luteal endothelial cells on endothelin-1 and prostaglandin F2a production.

牛黄体内皮细胞的储存和传代对内皮素-1 和前列腺素 F2a 产生的影响。

• 发表时间: 2007
• 期刊: Journal of Reproduction and Development 53, 3(in press)
• 作者: Acosta TJ;Yoshioka S;Komiyama J;Lee SH;Grazul-Bilska AT;Skarzynski DJ;Okuda K
• 通讯作者: Okuda K