Study on non-invasive measurement of biological redox for diagnosis of chronic diseases

1.酰基保护的羟胺探针(1-acetoxy-3-carbamoyl-2，2，5，5-tetramethylpyrrolidine：acp)在体内迅速水解形成羟胺，cPh。根据生物氧化还原状态的不同，预计CPH会转化为氮氧自由基，即CP。用ESR成像系统测量ACP给药后上腹部的ESR信号。在X射线照射的小鼠和脓毒症小鼠中，该信号都是持久的，而在健康的小鼠中，该信号减弱。健康和损伤小鼠肝脏中CP和CPH的总量无差异，说明ESR信号持续时间的不同反映了小鼠氧化还原状态的不同。结论：1.高血压小鼠的血沉信号持续时间明显延长。设计了一种含螺环已基环的新型氮氧基自旋探针。该探针具有较强的抗酶还原能力，而与抗坏血酸或氧自由基的反应活性与不含螺环的探针相似。

1. Acyl-protected hydroxylamine probe (1-acetoxy-3-carbamoyl-2,2,5,5-tetramethylpyrrolidine : ACP) is quickly hydrolyzed in a body to form hydroxylamine, CPH. It is expected that CPH is converted to nitroxyl radical, CP, depending on biological redox status. ESR signal was observed in the upper abdomen after administration of ACP, as measured by ESR imaging system. The signal was durable in both X-ray-irradiated mice and septic mice, while the signal decreased in healthy ones. There was no difference in the total amount of CP and CPH in the liver between healthy and injured mice, indicating that the difference in the duration of ESR signal reflects the difference in the redox status in mice. The duration of ESR signal was also observed in hypertensive mice.2. New nitroxyl spin probes with spirocyclohexyl rings were designed. The probes were resistant against enzymatic reduction, while their reactivity with ascorbic acid or oxygen radicals was similar to that of probes without spiro-rings.