Study on non-invasive measurement of biological redox for diagnosis of chronic diseases

生物氧化还原无创测量在慢性病诊断中的研究

• 批准号: 17590047
• 负责人: TAKESHITA Keizo
• 金额: $ 2.3万
• 依托单位: Sojo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590047/
• 关键词: Oxidative stress; Redox; ESR; Nitroxyl radical; Imaging; 酸化的ストレス; 両像解析

1. Acyl-protected hydroxylamine probe (1-acetoxy-3-carbamoyl-2,2,5,5-tetramethylpyrrolidine : ACP) is quickly hydrolyzed in a body to form hydroxylamine, CPH. It is expected that CPH is converted to nitroxyl radical, CP, depending on biological redox status. ESR signal was observed in the upper abdomen after administration of ACP, as measured by ESR imaging system. The signal was durable in both X-ray-irradiated mice and septic mice, while the signal decreased in healthy ones. There was no difference in the total amount of CP and CPH in the liver between healthy and injured mice, indicating that the difference in the duration of ESR signal reflects the difference in the redox status in mice. The duration of ESR signal was also observed in hypertensive mice.2. New nitroxyl spin probes with spirocyclohexyl rings were designed. The probes were resistant against enzymatic reduction, while their reactivity with ascorbic acid or oxygen radicals was similar to that of probes without spiro-rings.

1.酰基保护的羟胺探针（1-乙酰氧基-3-氨基甲酰基-2，2，5，5-四甲基吡咯烷：ACP）在体内快速水解形成羟胺，CPH。预期CPH转化为硝酰基自由基CP，这取决于生物氧化还原状态。应用ESR成像系统测量ACP给药后上腹部的ESR信号。这种信号在X射线照射的小鼠和脓毒症小鼠中都是持久的，而在健康的小鼠中信号下降。健康和损伤小鼠肝脏中CP和CPH的总量没有差异，表明ESR信号持续时间的差异反映了小鼠氧化还原状态的差异。观察高血压小鼠ESR信号持续时间.设计了一种新的含螺环己基环的硝酰基自旋探针。探针是抗酶的还原，而它们与抗坏血酸或氧自由基的反应性是类似的，没有螺环的探针。

项目成果

Enzymatic reduction-resistant nitroxyl spin probes with spirocyclohexyl rings

• DOI: 10.1080/10715760701449302
• 发表时间: 2007-01-01
• 期刊: FREE RADICAL RESEARCH
• 影响因子: 3.3
• 作者: Okazaki, Shoko;Mannan, Md Abdul;Takeshita, Keizo
• 通讯作者: Takeshita, Keizo

Hydroxyl Radical Generation via Photoreduction of a Simple Pyridine N-Oxide by an NADH Analogue.

通过 NADH 类似物光还原简单的吡啶 N-氧化物产生羟基自由基。

• 发表时间: 2005
• 期刊: Org. Biomol. Chem. 3・18
• 作者: Ikuo Nakanishi;et al.
• 通讯作者: et al.

Scandium ion-accelerated scavenging reaction of cumylperoxyl radical by a cyclic nitroxyl radical via electron transfer

• DOI: 10.1246/cl.2007.378
• 发表时间: 2007-03-05
• 期刊: CHEMISTRY LETTERS
• 影响因子: 1.6
• 作者: Nakanishi, Ikuo;Kawaguchi, Kumiko;Ikota, Nobuo
• 通讯作者: Ikota, Nobuo