Development, Analyze and Clinical Use of Tissue Engineering Large Diameter Autografts

组织工程大直径自体移植物的开发、分析和临床应用

• 批准号: 17591490
• 负责人: YAMAMOTO Noboru
• 金额: $ 2.37万
• 依托单位: Tokyo Women's Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591490/
• 关键词: Regenerative Medicine; Tissue Engineering; Tissue Engineered graft; Biodegradable Scaffold; Bone Marrow Cell; long term result; Endothelial Cell; growth factor; 大口径再生血管; ティッシュエンジニアリング; 生体吸収性素材; 動物実験n

This study demonstrates the evaluation of tissue-engineered vascular autografts (TEVAs) constructed with autologous mononuclear-bone marrow cells (MN-BMCs) and a biodegradable scaffold using a canine inferior vena cava (IVC) model.MN-BMCs were obtained from a dog and seeded onto a biodegradable tubular scaffold consisting of polyglycolide fiber and poly(1-lactide-co-ε-caprolactone) sponge. This scaffold was implanted in the IVC of the same dog on the day of surgery. TEVAs were analyzed biochemically, biomechanically and histologically after implantation. When TEVAs were explanted and stimulated with acetylcholine at 1 month, they dose dependently produced nitrates and nitrites. N^G-nitro-L-arginine methylester significantly inhibited these reactions. On the stimulation by acetylcholine, factor VIII-positive cells of TEVAs produced eNOS proteins, and the ratio of eNOS/s17 mRNA were similar among native IVC, 1-and 3-months TEVAs. TEVAs had similar biochemical properties and wall thickness as native IVC at 6 months after implantation, and tolerated venous pressure well without any problems such as calcification.These results indicate that TEVAs are biocompatible material with functional endothelial cells and biomechanical properties without unwanted side-effects.

本研究采用犬下腔静脉（IVC）模型，将自体骨髓单核细胞（MN-BMCs）接种于由聚乙交酯纤维和聚（1-丙交酯-co-ε-己内酯）海绵组成的可生物降解管状支架上，构建组织工程化自体血管（TEVA）。在手术当天将该支架植入同一只狗的IVC中。植入后对TEVA进行生物化学、生物力学和组织学分析。当TEVA在1个月时用乙酰胆碱刺激和刺激时，它们剂量依赖性地产生硝酸盐和亚硝酸盐。N^G-硝基-L-精氨酸甲酯显着抑制这些反应。在乙酰胆碱的刺激下，TEVA的因子VIII阳性细胞产生eNOS蛋白，并且eNOS/s17 mRNA的比率在天然IVC、1个月和3个月TEVA之间相似。TEVA植入后6个月的生物化学性质和壁厚与天然IVC相似，并且耐受静脉压力良好，没有任何问题，如钙化。这些结果表明TEVA是具有功能性内皮细胞和生物力学性质的生物相容性材料，没有不良副作用。

项目成果

A successful total cavopulmonary connection conversion 13 years after anatomic repair for tricuspid atresia IIc with severe pulmonary resistance

具有严重肺阻力的三尖瓣闭锁 IIc 解剖修复术后 13 年成功完成全腔肺连接转换

• 发表时间: 2005
• 期刊: J Thorac Cardiovasc Surg 129
• 作者: Sugimoto K;Kurosawa H;Shin'oka T;Nagatsu M;Morishima S
• 通讯作者: Morishima S

Mid-term clinical results of tissue engineered vascular autografts seeded with autologous bome marrow cells

自体骨髓细胞接种组织工程自体血管移植物的中期临床结果

• 发表时间: 2005
• 期刊: J Thorac Cardiovasc Surg 129
• 作者: Shin'oka T;Matsumura G;Hibino N;Naito Y;Watanabe M;Konuma T;Sakamoto T;Nagatsu M;Kurosawa
• 通讯作者: Kurosawa

培養人工血管-Tissue Engineering Graft-

培养人工血管-组织工程移植物-

• 发表时间: 2006
• 期刊: 分子心血管病 7(5)
• 作者: Toshiki Watanabe;MD;Sadao Omata;PhD;Motoki Odamura;PhD;Masahumi Okada;Phd;Yoshihiko Nakamura;PhD;Hitoshi Yokoyama;MD;Matsumura Goki;宮本 真嘉;市原 有起;保々 恭子
• 通讯作者: 保々 恭子

Autopericardial patch tracheoplasty for tracheal stenosis after arterial switch operation

自体心包补片气管成形术治疗动脉调转术后气管狭窄

• 发表时间: 2005
• 期刊: J Card Surg 20
• 作者: Takiguchi M;Aoki M;Shin'oka T;Hiramatsu T;Imai Y
• 通讯作者: Imai Y

The tissue-engineered vascular graft using bone marrow without culture

• DOI: 10.1016/j.jtcvs.2004.10.030
• 发表时间: 2005-05-01
• 期刊: JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY
• 影响因子: 6
• 作者: Hibino, N;Shin'oka, T;Kurosawa, H
• 通讯作者: Kurosawa, H