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Tissue Engineered Vessels and Valve constructed with Autologous Bone Marrow Cells Its Clinical Applications and Evaluations

自体骨髓细胞构建的组织工程血管和瓣膜的临床应用和评价

基本信息

批准号：
17390387
负责人：
YAMAMOTO Noboru
金额：
$ 9.7万
依托单位：
Tokyo Women's Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

Prosthetic and bioprosthetic materials currently in use lack growth potential and therefore must be repeatedly replaced in pediatric cardiovascular patients as they grow. Tissue engineering is a new discipline that offers the potential for creating replacement structures from autologous cells and biodegradable scaffolds. In May 2000, we initiated clinical application of tissue-engineered vascular autografts (TEVAs) seeded with cultured cells. However, cell culturing is time-consuming, and xenoserum must be used. To overcome these disadvantages, we began to use bone marrow cells, readily available on the day of surgery, as a cell source.Since September 2001, 44 patients had a cardiothoracic operation using TEVAs seeded with autologous bone marrow cells. Three to 10 mL/kg specimen of bone marrow was aspirated with the patient under general anesthesia before the skin incision. These cells were seeded onto the absorbable scaffold before implantation. This scaffold was composed of a copolym … More er of P (CL/LA) which degrades by hydrolysis. PLLA or PGA woven fabric were used for reinforcement of the scaffolds. Twenty-six tissue-engineered conduits and 19 tissue-engineered patches were used for the repair of congenital heart defects. Patients who had undergone this method had a catheterization study, CT scan, or both for the evaluation after the operation. Four late deaths due to the heart failure with or without multiple organ failure or brain bleeding in this series; these were unrelated to the TEVAs function. A few patient required PTA for the tubular graft-stenosis with pressure gradient and / or collaterals, and four patients for the stenosis of the tissue-engineered patch. However, this tissue-engineering approach may provide an important alternative to the use of prosthetic materials in the field of pediatric cardiovascular surgery. As it utilizes living autologous living tissue, this newly developed vascular structure may have the potential to achieve "auto-tissue repair" for the children with congenital heart defects. However, this approach is still in its infancy, further experimental studies to resolve the problems presented, and longer follow-up in patients are necessary to confirm the durability of this approach. Less

目前使用的假体和生物假体材料缺乏生长潜力，因此必须随着儿童心血管患者的生长而反复更换。组织工程是一门新兴学科，它提供了从自体细胞和可生物降解支架中创造替代结构的可能性。2000年5月，我们开始了组织工程化血管自体移植物（TEVA）的临床应用与培养细胞的种植。然而，细胞培养是耗时的，并且必须使用异种血清。为了克服这些缺点，我们开始使用骨髓细胞，在手术当天容易获得，作为细胞来源。自2001年9月以来，44名患者进行了心胸手术，使用TEVA与自体骨髓细胞接种。在皮肤切开前，患者在全身麻醉下抽吸3 - 10 mL/kg的骨髓样本。在植入前将这些细胞接种到可吸收支架上。这种支架由一种共聚物组成， ...更多信息水解降解的P（CL/LA）。PLLA或PGA编织织物用于支架的增强。26个组织工程管道和19个组织工程补片用于先天性心脏病的修复。接受这种方法的患者在手术后进行导管检查，CT扫描或两者兼而有之以进行评估。该系列中有4例因心力衰竭伴或不伴多器官衰竭或脑出血而晚期死亡;这些死亡与TEVA功能无关。少数患者需要PTA治疗存在压力梯度和/或侧支循环的管状移植物狭窄，4例患者需要PTA治疗组织工程补片狭窄。然而，这种组织工程方法可能提供一个重要的替代使用的假体材料在儿科心血管外科领域。由于它利用了活体自体组织，这种新开发的血管结构可能有潜力实现对先天性心脏缺陷儿童的“自体组织修复”。然而，这种方法仍处于起步阶段，需要进一步的实验研究来解决所提出的问题，并对患者进行更长时间的随访以确认这种方法的持久性。少

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

培養人工血管-Tissue Engineering Graft-

培养人工血管-组织工程移植物-

DOI：
发表时间：
2006
期刊：
分子心血管病 7(5)
影响因子：
0
作者：
Toshiki Watanabe;MD;Sadao Omata;PhD;Motoki Odamura;PhD;Masahumi Okada;Phd;Yoshihiko Nakamura;PhD;Hitoshi Yokoyama;MD;Matsumura Goki;宮本真嘉;市原有起;保々恭子
通讯作者：
保々恭子

The tissue-engineered vascular graft using bone marrow without culture

DOI：
10.1016/j.jtcvs.2004.10.030
发表时间：
2005-05-01
期刊：
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY
影响因子：
6
作者：
Hibino, N;Shin'oka, T;Kurosawa, H
通讯作者：
Kurosawa, H

Mid-term clinical results of tissue engineered vascular autografts seeded with autologous bome marrow cells

自体骨髓细胞接种组织工程自体血管移植物的中期临床结果

DOI：
发表时间：
2005
期刊：
J Thorac Cardiovasc Surg 129
影响因子：
0
作者：
Shin'oka T;Matsumura G;Hibino N;Naito Y;Watanabe M;Konuma T;Sakamoto T;Nagatsu M;Kurosawa
通讯作者：
Kurosawa

自己骨髄細胞と生体吸収性ポリマーにより作製した再生血管による臨床研究44症例とその経過

自体骨髓细胞与生物可吸收聚合物再生血管的44项临床研究及其进展