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Experimental study of tendon adhesion prevention by a biocompatible polymer hydrogel.

生物相容性聚合物水凝胶预防肌腱粘连的实验研究。

基本信息

批准号：
17591548
负责人：
MORO Toru
金额：
$ 2.18万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591548/
关键词：
surgery tendon injury anti-adhesion material biomaterial biocompatibility polymer hydrogel a high molecular material

项目摘要

2-methacryloyloxyethyl phosphorylcholine(MPC) polymer is our original biocompatible polymer whose side chain is composed of phosphorylcholine that resembles phospholipids of biomembrane. The MPC hydrogel can be generated by mixing aqueous solutions of poly (MPC-co-methacrylic acid)(PMA) and poly(MPC-co-n-butyl methacrylate)(PMB). The present study investigated the effects of the MPC-hydrogel application on tendon adhesion and healing.1.Determination of the optimum condition for synthesis of MPC hydrogelVarious gels were generated by changing the molecular composition, molecular weight, structure of hydrophobic units, and evaluated in simulated body fluid and using rat model. The established optimal gel was used for the following studies 2-4.2. Investigation of the effects of MPC hydrogel for tendon healing..After a tendon was cut and sutured, the MPC hydrogel was applied to cover the injury site and the wound was closed.Tendon healing was evaluated by macroscopic, histological and mechanical testings.Macroscopic findings showed little or no adhesion was observed in the MPC group.HE staining showed that gaps of the injured tendon in both groups were similarly bridged by collagen fibers.There was no significant difference on tensile strength between two groups.3. Investigation of the effects of MPC hydrogel for prevention of tendon adhesion.After the same manner, tendon adhesion was evaluated by microscopic and mechanical testings.The findings showed severe adhesion in the control group. In contrast, little or no adhesion was observed in the MPC group.4. Evaluation of the lubrication property of MPC hydrogel covering a tendon suture site.By measuring of the tendon excursion resistance after cyclic flexion of the operated toe, the excellent lubrication property of MPC hydrogel was revealed.In conclusion, the MPC-hydrogel application immediately after the tendon suture may prevent the adhesion without impairing the healing.

2-甲基丙烯酰氧乙基磷酸胆碱（MPC）聚合物是我们最初的生物相容性聚合物，其侧链由类似生物膜磷脂的磷酸胆碱组成。MPC水凝胶是由聚MPC-共甲基丙烯酸（PMA）和聚MPC-共甲基丙烯酸正丁酯（PMB）水溶液混合制备的。本研究探讨了mpc -水凝胶对肌腱粘附和愈合的影响。通过改变疏水单元的分子组成、分子量、结构来制备各种凝胶，并在模拟体液和大鼠模型中进行评价。建立的最佳凝胶用于以下研究2-4.2。MPC水凝胶对肌腱愈合效果的研究。在肌腱切开缝合后，应用MPC水凝胶覆盖损伤部位并关闭伤口。通过宏观、组织学和力学测试评估肌腱愈合情况。宏观结果显示MPC组很少或没有粘连。HE染色显示两组损伤肌腱间隙均以胶原纤维桥接。两组间抗拉强度无显著差异。MPC水凝胶预防肌腱粘连效果的研究。以同样的方法，通过显微和力学测试评估肌腱粘附性。结果显示对照组粘连严重。相比之下，MPC组很少或没有观察到粘连。覆盖肌腱缝合部位的MPC水凝胶润滑性能的评价。通过对足趾循环屈曲后肌腱偏移阻力的测定，揭示了MPC水凝胶具有优异的润滑性能。总之，在肌腱缝合后立即应用mpc -水凝胶可以防止粘连而不影响愈合。