Nano-particles which Contained Drug Induction into Tumor Cell by Electrochemotherapy and Sonoporation

含有药物的纳米颗粒通过电化学疗法和声孔法诱导进入肿瘤细胞

基本信息

批准号：
17591559
负责人：
TAKAHASHI Jun
金额：
$ 2.24万
依托单位：
Shinshu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591559/
关键词：
nano-particle sonoporation drug delivery system anti cancer drug 電気刺激ドラッグデリバリー

项目摘要

1. Manufacture of nano-particles which contained an anti cancer drugWe made polypeptide [ (GVGVP) 25] besides the polypeptide Poly [ (GVGVP GVGVP GKGVP GVGVP GVGVP GVGVP)_<22>(GVGVP)] which made in last year and impregnated red dyes (Brebrich Scarlet Red) in the [ (GVGVP) 25] particle. Pigments were gradually discharged from them more than two weeks. We let 3. 5mg methotrexate fuse for the 30mg peptide. As a result, MTX was released more than minimum 3 days.2 Nano-particles conjugated anticancer drug and anticancer drug induction into tumor cell by sonoporation and enhanced antitumor effectsWe examined whether enhancement of the antitumor effect of sonoporation was feasible by using nano-particle of methotrexate component in mice Lewis lung carcinoma. The tumor bearing mice were divided into five groups : (1) no treatment (2) methotrexate treatment alone (3) nano-particle of methotrexate component treatment alone (4) methotrexate treatment followed by sonoporation(5)nano-particle of methotrexate component treatment followed by sonoporation. Sonoporation was applied to the tumors after intraperitoneal injection of drugs. Tumors of the mice were measured every 2 days after treatment. Differences in the rate of tumor volume increase between No. (4), (5) groups and other three groups were significant. As for No. (5) group in particularly, antitumor effect was enhanced. The methotrexate content of the tumor tissues from the sonoporation groups (No. (4), (5)) were higher than other groups. To confirm the promotion of methotrexate induction in vivo tumor cells by sonoporation, fluorescence conjugated MTX was administered into tumor cell. In the presence of sonoporation, fluorescence-positive cells were observed, while only a weakly positive area was observed in the absence of sonoporation.

1。除多肽Poly [（GVGVP GVGVP GKGVP GKGVP GVGVP GVGGVP GVGGVP GVGGVP）外，含有抗癌药物的纳米颗粒制造了多肽[（GVGVP）25]。（GVGVP）25]粒子。颜料逐渐从它们中排出超过两个星期。我们将3.5mg甲氨蝶呤融合用于30mg肽。结果，MTX的释放超过3天以上。2纳米粒子通过超声层构造抗癌药物和抗癌药物通过超声层诱导诱导到肿瘤细胞中，并增强了抗肿瘤效应，检查了使用甲基甲酸甲酸盐组成的nano-partice in Mecexate in Mice-carcina sonotial sonoporation sonoporation of Sonoporation的增强是否可行。将肿瘤轴承小鼠分为五组：（1）单独使用甲氨蝶呤治疗（3）单独的甲氨蝶呤成分治疗的纳米粒子（4）甲氨蝶呤治疗，然后进行超声处理（5）甲氨蝶呤成分治疗的纳米粒子，然后进行超声处理。腹膜内注射药物后，将其用于肿瘤。治疗后每2天每2天测量小鼠的肿瘤。（4），（5）组和其他三组之间的肿瘤体积率差异很大。至于第（5）个组，尤其是抗肿瘤效应。来自Sonoporation组的肿瘤组织的甲氨蝶呤含量（第（4），（5））高于其他组。为了确认通过超声层促进体内肿瘤细胞中甲氨蝶呤的诱导，将荧光共轭MTX施用到肿瘤细胞中。在存在超声的情况下，观察到荧光阳性细胞，而在没有超声的情况下仅观察到弱的正区域。