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Nano-particles which Contained Drug Induction into Tumor Cell by Electrochemotherapy and Sonoporation

含有药物的纳米颗粒通过电化学疗法和声孔法诱导进入肿瘤细胞

基本信息

批准号：
17591559
负责人：
TAKAHASHI Jun
金额：
$ 2.24万
依托单位：
Shinshu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591559/
关键词：
nano-particle sonoporation drug delivery system anti cancer drug 電気刺激ドラッグデリバリー

项目摘要

1. Manufacture of nano-particles which contained an anti cancer drugWe made polypeptide [ (GVGVP) 25] besides the polypeptide Poly [ (GVGVP GVGVP GKGVP GVGVP GVGVP GVGVP)_<22>(GVGVP)] which made in last year and impregnated red dyes (Brebrich Scarlet Red) in the [ (GVGVP) 25] particle. Pigments were gradually discharged from them more than two weeks. We let 3. 5mg methotrexate fuse for the 30mg peptide. As a result, MTX was released more than minimum 3 days.2 Nano-particles conjugated anticancer drug and anticancer drug induction into tumor cell by sonoporation and enhanced antitumor effectsWe examined whether enhancement of the antitumor effect of sonoporation was feasible by using nano-particle of methotrexate component in mice Lewis lung carcinoma. The tumor bearing mice were divided into five groups : (1) no treatment (2) methotrexate treatment alone (3) nano-particle of methotrexate component treatment alone (4) methotrexate treatment followed by sonoporation(5)nano-particle of methotrexate component treatment followed by sonoporation. Sonoporation was applied to the tumors after intraperitoneal injection of drugs. Tumors of the mice were measured every 2 days after treatment. Differences in the rate of tumor volume increase between No. (4), (5) groups and other three groups were significant. As for No. (5) group in particularly, antitumor effect was enhanced. The methotrexate content of the tumor tissues from the sonoporation groups (No. (4), (5)) were higher than other groups. To confirm the promotion of methotrexate induction in vivo tumor cells by sonoporation, fluorescence conjugated MTX was administered into tumor cell. In the presence of sonoporation, fluorescence-positive cells were observed, while only a weakly positive area was observed in the absence of sonoporation.

1.含有抗癌药物的纳米粒子的制备我们在去年合成的多肽[(GVGVP)GVGVP GKGVP GVGVP GVGVP GVGVP)_&lt；22&gt；(GVGVP)的基础上制备了多肽[(GVGVP)25]，并在[(GVGVP)25]粒子中浸渍了红色染料(布里奇红)。两个多星期后，色素逐渐排出。我们让3.5毫克的甲氨蝶呤熔合成30毫克的多肽。结果MTX的释放量至少在3天以上。2.纳米粒子结合抗癌药物和抗癌药物通过声学修饰进入肿瘤细胞并增强抗肿瘤作用。我们考察了利用纳米甲氨蝶呤成分增强抗癌作用的可行性。将荷瘤小鼠分为5组：(1)不治疗组(2)单纯甲氨蝶呤治疗(3)单纯甲氨蝶呤纳米颗粒治疗(4)甲氨蝶呤+声孔修复(5)甲氨蝶呤纳米颗粒+声孔修复。肿瘤经腹膜腔注药后行声学造影术。治疗结束后每隔2天测量一次小鼠肿瘤体积。两组肿瘤体积增加率的差异有统计学意义。(4)、(5)组和其他三组差异有统计学意义。至于No.(5)各剂量组抗肿瘤作用增强。超声照射组肿瘤组织中甲氨蝶呤含量(No.(4)、(5))高于其他组。将荧光标记的甲氨蝶呤(MTX)注入肿瘤细胞内，以证实超声修饰对体内肿瘤细胞的诱导作用。在声孔作用下，观察到荧光阳性的细胞，而在没有声孔作用的情况下，只观察到一个弱阳性的区域。