RFT gene expression in malignant transformation of gliomas

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The regulator of fibroblast growth factor (FGF)-2 transcription (RFT) has been, reported to be a transcriptional repressor of FGF-2 and induce glioma cell death by its overexpression. Here we report that RFT regulated cell cycle as well as apoptosis by a novel mechanism. RFT expressed in some glioma cell lines, U 13 8MG and T98G, but neither in U25iMG and U87MG Over expressed RFT induced apoptosis in U87MG and U138MG with functioning-type p53 but neither in U251Mg nor T98G with non-functioning-type p53. Administration of FGF-2 failed to prevent RFT induced apoptosis. Overexpression of RFT caused GI-S arrest and upregulated buts the phosphorylation of p53 at Ser-15 and the expression level of p2lwafl. Furthermore, RNAi knockdown of p53 abolished RFT induced apoptosis in U87MG. Taken together, our results support that RFT regulates Gl-S transition and apoptosis via p53/p2lwafl pathway.