Clarification of the roles of SDF-1 on mesenchymal stem cells in bone graft healing-toward a clinical application for the improvement of allograft healing-

阐明SDF-1对间充质干细胞在骨移植愈合中的作用-面向改善同种异体移植愈合的临床应用-

• 批准号: 17591564
• 负责人: ITO Hiromu
• 金额: $ 2.3万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591564/
• 关键词: mesenchymal stem cell; bone transplantation; bone formation; SDF-1; 移植、再生治療

sDF-1 and CXCR4 were constitutively expressed by primary rBMSCs during osteoblastic and chondrocytic differentiation. The expression of SDF-1 mRNA was increased by 3.1 folds in the autograft healing at day 2 after surgery compared with that in the allograft model. Immunohistochemical analysis revealed the high expression of SDF-1 protein in the periosteum of the autografts. While the gain of function studies showed no remarkable increase in the bone formation, both the new bone formation around the autograft and the recruitment of MSCs were significantly inhibited by the administration of anti-SDF-1 antibody. SDF-1 upregulated the in vitro migration of rBMSCs in a dose dependent manner, and the effect was inhibited by TN 14003, an antagonist for CXCR4. These results indicate that SDF-1 has a chemotactic effect on BMSCs both in vitro and in vivo and plays a crucial role in the acute phase of normal bone repair. However, these results also suggest that SDF-1 may need to collaborate with other molecules to achieve a successful bone repair.

sDF-1和CXCR 4在原代rBMSCs向成骨细胞和软骨细胞分化过程中呈组成性表达。术后第2天，自体移植骨愈合中SDF-1 mRNA的表达较同种异体移植骨增加3.1倍。免疫组织化学分析显示SDF-1蛋白在自体移植物骨膜中的高表达。虽然功能的获得研究显示骨形成没有显著增加，但抗SDF-1抗体的施用显著抑制了自体移植物周围的新骨形成和MSC的募集。SDF-1以剂量依赖性方式上调rBMSCs的体外迁移，这种作用可被CXCR 4拮抗剂TN 14003抑制。这些结果表明，SDF-1在体外和体内对BMSCs具有趋化作用，并在正常骨修复的急性期起着至关重要的作用。然而，这些结果也表明SDF-1可能需要与其他分子合作才能实现成功的骨修复。

项目成果

Periosteal progenitor cell fate in segmental cortical bone graft transplantations

节段性皮质骨移植中骨膜祖细胞的命运

• 发表时间: 2005
• 期刊: J Bone Miner Res. 20(12)
• 作者: Zhang X;Xie C;Lin AS;Ito H et al.
• 通讯作者: Ito H et al.

Periosteal progeitor cell fate in segmental cortical bone graft transplantation : implications for functional tissue engineering

节段性皮质骨移植中的骨膜祖细胞命运：对功能组织工程的影响

• 发表时间: 2005
• 期刊: J Bone Miner Res. 20(12)
• 作者: Zhang X.;et al.
• 通讯作者: et al.

Periosteal progenitor cell fate in segmental cortical bone graft transplantations: Implications for functional tissue engineering

• DOI: 10.1359/jbmr.050806
• 发表时间: 2005-12-01
• 期刊: JOURNAL OF BONE AND MINERAL RESEARCH
• 影响因子: 6.2
• 作者: Zhang, XP;Xie, C;Guldberg, RE
• 通讯作者: Guldberg, RE