Relationship between abnormal expression of transcription genes and functional alteration of cancer cells in gynecologic cancers

结果：1、反义HOXB 7基因转染SKOV 3细胞后，细胞侵袭能力在16 h时即被完全抑制，而单个反义HOXB 7基因转染SKOV 3细胞后，细胞侵袭能力未被完全抑制; 2、反义HOXB 7基因转染SKOV 3细胞后，β-catenin和E-cadherin表达上调2 ~ 3倍。将HOXB 13反义导入SKOV 3后，E-钙粘蛋白的表达量增加了2倍至18倍。β-catenin和E-cadherin可能参与HOX基因在卵巢癌中的侵袭过程。3、HOXB 13、HOXC 11、HOXC 13基因在子宫内膜癌细胞中的表达谱显示为过表达，而在正常组织中均未观察到这些基因的表达，表明这些基因在子宫内膜癌的发生中起重要作用。反义HOXB 13基因的导入可显著降低子宫内膜癌细胞对原癌细胞的侵袭能力（90%），提示HOXB 13基因的过表达在子宫内膜癌的侵袭和/或转移中起重要作用。在10 μ M 17β-雌二醇处理后，HOXB 13表达的定量标准化为β-肌动蛋白<-5>。HOXB 13的表达稳步增加，在10小时达到高峰。HOXB 13在子宫内膜癌中的表达可能受雌激素的时间和剂量依赖性调节。5、DLX 1在卵巢癌细胞和组织中有表达，而在正常卵巢上皮中无表达。TBX 1在子宫内膜癌细胞和组织中有表达，而在正常子宫内膜中无表达。

1, The complete suppression of the cell invasion by the multiple introducing antisenses into SKOV3 was observed at the time course of sixteen hours although the single antisense introduced cells did not showed the complete suppression of its invasion.2, The introduction of antisense HOXB7 in SKOV3 cell showed the up-regulation of β-catenin and E-cadherin in two fold to three fold. The antisense introduced HOXB13 into SKOV3 demonstrated that the high level of expression of E-cadherin in two fold to eighteen folds. β-catenin and E-cadherin are suggested as the factors indicating the possibility of the effect for the process of the invasion involving HOX gene in ovarian cancer.3, Expression profile of endometrial cancer cells demonstrated overexpression in HOXB13,HOXC11,HOXC13 genes, whereas no expression in normal tissues was observed in those genes, indicating these genes are important for endometrial carcinogenesis.4, Antisense introduction of overexpressed HOXB13 gene showed marked reduction (90%) of invasion ability to original cancer cells, suggesting overexpression of HOXB13 gene plays an important role in tumor invasion and/or metastasis in endometrial cancer. Quantification of HOXB13 expression normalized to β-actin after 10^<-5>M 17β-estradiol treatment. The expression of HOXB13 increased steadily, reaching a peak at 10 hours. HOXB13 expression in endometrial caner is possible to be regulated by estrogens with time and dose dependent manner.5, The expression of DLX1 was detected in ovarian carcinoma cells and tissues, whereas it was not detected in normal epithelium of ovary. The expression of TBX1 was detected in endometrial carcinoma cells and tissues, whereas it was not detected in normal endometrium.