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Role of nitric oxide in facial nerve paralysis in mice induced by herpes simplex type 1 infection

一氧化氮在 1 型单纯疱疹病毒感染小鼠面神经麻痹中的作用

基本信息

批准号：
17591791
负责人：
HATO Naohito
金额：
$ 2.24万
依托单位：
Ehime University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591791/
关键词：
Bell' s palsy facial nerve paralysis HSV-1 NO iNOS edarabone 単純ヘルペスウイルス脱髄

项目摘要

The present study was under gone to elucidate a role of nitric oxide (NO) on induction of facial palsy by inoculation of herpes simplex type 1 (HSV-1), and to evaluate the effects of edarabone to prevent occurrence of facial palsy of the viral origin. NO levels of in the facial nerve were measured by use of a high performance liquid chromatography and absorption photometry. Before the incidence of facial palsy, no substantial difference in NO levels was noted between HSV-1 inoculated sides (right) and the control (left) sides. When the facial nerve palsy occurred, NO levels of the paralyzed side were higher than of the other side. Following recovery of the palsy, NO of both sides were at the same level. In the mice that facial nerve paralysis did not occur in spite of inoculation of HSV-1, NO of both sides were at the same level. According to immunohistological study using the confocal laser microscope, inducible nitric oxide synthase (iNOS) was observed in the inflammatory cells at the geniculate ganglion in animals with facial palsy presents. It was recognized only at the involved paralyzed side not at the contralateral side. Edarabone, a free radical scavenger, was administed to the animals immediately after inoculation of HSV-1 for as long as 11 days. The results indicated that 10mg/kg of edarabone was effective in reducing the incidence of facial palsy. In conclusion, NO, which is supposed to be produced at the geniculate ganglion via iNOS, plays an important role in induction of facial palsy by HSV-1, and, such damages may be prevented by use of edarabone.

本研究旨在阐明一氧化氮（NO）在单纯疱疹病毒1型（HSV-1）诱发面神经麻痹中的作用，并评价依达拉奉预防病毒性面神经麻痹的作用。采用高效液相色谱法和分光光度法测定面神经组织中NO含量。在发生面瘫之前，在HSV-1接种侧（右）和对照侧（左）之间没有观察到NO水平的实质性差异。面神经麻痹时，麻痹侧NO水平高于对侧。瘫痪恢复后，双侧NO水平相同。接种HSV-1后未发生面神经麻痹的小鼠，双侧面神经NO水平相同。应用激光共聚焦显微镜观察面神经麻痹动物膝状体神经节内的炎性细胞中诱导型一氧化氮合酶（inducible nitric oxide synthase，iNOS）的表达。仅在受累瘫痪侧识别，对侧无识别。在接种HSV-1后立即给予自由基清除剂依达拉朋长达11天。结果表明，依达拉奉10 mg/kg能有效降低面神经麻痹的发生率。总之，一氧化氮，这是应该通过诱导型一氧化氮合酶在膝状神经节产生，在诱导面神经麻痹的HSV-1中起着重要作用，并且，这种损害可以通过使用依达拉奉来预防。