RNA expression in facial muscle following damage to the facial nerve -As a tool of early prognostic diagnosis-

本研究对面神经损伤大鼠面部肌肉差异表达基因进行了研究和鉴定。两种类型的成年Wistar大鼠：1)切断面神经主干(A组)和2)主干压迫10分钟(B组)用于分析。使用微阵列系统分析面部肌肉的整体基因表达。总共检查了26,835个RNA分子。A组失神经后7d肌肉生成素mRNA表达达到最高水平，随后逐渐下降，A组在7d和14d表达增加，基因表达随面神经损伤程度的不同而不同。因此，我们认为，面神经损伤后面肌轮廓基因表达的分析可能是一种可能用于面神经损伤后预后的诊断工具。

This study investigated and identified differentially expressed facial muscle genes in rats with facial nerve damage. Two types of adult Wistar rats: 1) the main trunk of the facial nerve was transected(Group A) and 2) the main trunk was compressed for ten minutes (Group B) wereused for the analysis. Global gene expressions of facial muscles were analyzed using a micro-array system. A total of 26,835 RNA molecules were examined. Myogenin mRNA expression increased to the highest level at 7 days after denervation, thereafter it gradually decreased in Group A. Vesicle-associated membrane protein 2 and insulin-like growth factor binding protein 6 expression increased at 7 and 14 days in Group A. The gene expression was different depending on the degree of the facial nerve damage. Therefore, we concluded that analysis of the facial muscle profile gene expression could be a possible diagnostic tool for use in asthe prognosis after facial nerve injury.