RNA expression in facial muscle following damage to the facial nerve -As a tool of early prognostic diagnosis-

面神经损伤后面部肌肉中的 RNA 表达 -作为早期预后诊断的工具 -

• 批准号: 22591884
• 负责人: HATO Naohito
• 金额: $ 2.83万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591884/
• 关键词: 顔面神経; 神経挫滅; マイクロアレイ法; RNA解析; 神経切断

This study investigated and identified differentially expressed facial muscle genes in rats with facial nerve damage. Two types of adult Wistar rats: 1) the main trunk of the facial nerve was transected(Group A) and 2) the main trunk was compressed for ten minutes (Group B) wereused for the analysis. Global gene expressions of facial muscles were analyzed using a micro-array system. A total of 26,835 RNA molecules were examined. Myogenin mRNA expression increased to the highest level at 7 days after denervation, thereafter it gradually decreased in Group A. Vesicle-associated membrane protein 2 and insulin-like growth factor binding protein 6 expression increased at 7 and 14 days in Group A. The gene expression was different depending on the degree of the facial nerve damage. Therefore, we concluded that analysis of the facial muscle profile gene expression could be a possible diagnostic tool for use in asthe prognosis after facial nerve injury.

本研究对面神经损伤大鼠面部肌肉差异表达基因进行了研究和鉴定。两种类型的成年Wistar大鼠：1)切断面神经主干(A组)和2)主干压迫10分钟(B组)用于分析。使用微阵列系统分析面部肌肉的整体基因表达。总共检查了26,835个RNA分子。A组失神经后7d肌肉生成素mRNA表达达到最高水平，随后逐渐下降，A组在7d和14d表达增加，基因表达随面神经损伤程度的不同而不同。因此，我们认为，面神经损伤后面肌轮廓基因表达的分析可能是一种可能用于面神经损伤后预后的诊断工具。

项目成果

Bell 麻痺に対する再生促進顔面神経減荷手術

面神经减负手术促进贝尔麻痹再生

• 发表时间: 2012
• 作者: 羽藤直人;能田淳平;菰渕勇人;澤井尚樹;寺岡正人;暁 清文
• 通讯作者: 暁 清文

Myogenin expression in facial muscle following damage to the facial nerve

• DOI: 10.3109/00016489.2011.653671
• 发表时间: 2012-06
• 期刊: Acta Oto-Laryngologica
• 影响因子: 1.4
• 作者: M. Teraoka;N. Hato;Hirotaka Takahashi;Hayato Komobuchi;Naoki Sawai;Masahiro Okada;N. Hakuba

側頭骨骨折への対応:顔面神経障害を中心に

颞骨骨折的治疗：重点关注面神经疾病

• 发表时间: 2010
• 作者: 豊田英樹;他;羽藤直人
• 通讯作者: 羽藤直人

Bell 麻痺、Hunt 症候群

贝尔氏麻痹症、亨特综合症

• 发表时间: 2010
• 期刊: Clinical Neuroscience
• 作者: 菅原一真;山下裕司;羽藤直人
• 通讯作者: 羽藤直人

Delayed Decompression Surgery for Severe Facial Nerve Paralysis using bFGF in a Gelatin Hydrogel

使用明胶水凝胶中的 bFGF 进行延迟减压手术治疗严重面神经麻痹

• 发表时间: 2012
• 作者: Hato N MD;Nota J MD;Komobuchi H MD;Teraoka M MD;Yamada H MD;Gyo K MD;Yanagihara N MD and Tabata Y* PhD
• 通讯作者: Yanagihara N MD and Tabata Y* PhD