Application of Immobilized Cytokine for Regeneration Medicine in Head and neck Region

固定化细胞因子在头颈部再生医学中的应用

• 批准号: 17591790
• 负责人: TSUJIGIWA Hidetsugu
• 金额: $ 2.11万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591790/
• 关键词: Immobilization; BMP; Scaffold; Collagen; Microenvironment; BMP-2; アテロコラーゲン; 幹細胞; 再生医学; 生体材料; サイトカイン; rhBMP-2

In the present study, we evaluated immobilized cytokine as a future therapy for tissue regeneration in head and neck region. The use of rhBMP-2 to induce ectopic bone formation requires a carrier. Type I atelocollagen is a biomaterial with a porous structure, excellent operational features and biocompatibility, which is an effective carrier for rhBMP-2. However, rhBMP-2/type I atelocollagen mixture does not necessarily give adequate bone induction effect. Because of this, we evaluated the effect of immobilizing rhBMP-2 to succinylated type I atelocollagen on the cellular activity of ST2, C2C12, MC3T3 cells in vitro study. Moreover, immobilized rhBMP-2/atelocollagen and non-immobilized rhBMP-2/atelocollagen were implanted in subcutaneous pockets of Wister rats. Our results indicated that 1) Alkaline phosphatase activity confirmed the effectiveness of rhBMP-2/ succinylated type I atelocollagen immobilization in augmenting cellular activity. 2) Intracellular signaling continued for prolon … More ged period of time when rhBMP-2 was immobilized to succinylated type I atelocollagen. 3) In rhBMP-2/atelocollagen implants showed higher osteoblast activity than non-immobilized rhBMP-2 in vivo.In order to use immobilized cytokine scaffold for bone formation, we firstly examined the efficacy of atelocollagen honeycomb scaffold for stem cell. An example of stem cell is KUSA/A1 cell. KUSA/A1 cells with atelocollagen and without atelocollagen were implanted of SCID mice. Both KUSA/A1 cells alone and KUSA/Al-Atelocollagen showed some radiopaque areas formation but the latter disclosed a larger amount. Histologically, KUSA/A1 cells alone showed few small islands of new bone formation surrounded by a thin layer of cellular proliferation. On the other hand, KUSA/Al-Atelocollagen revealed abundant new bone formation as well as cellular proliferation. This results support that atelocollagen honeycomb scaffold plays an important role in cellular anchorage and in vessel invasion, giving the precise shape and size for the new bone formation. Less

在本研究中，我们评估了固定化细胞因子作为头颈部组织再生的未来疗法。使用rhBMP-2诱导异位骨形成需要载体。I型去端胶原是一种具有多孔结构、良好操作性能和生物相容性的生物材料，是rhBMP-2的有效载体。然而，rhBMP-2/I型去端胶原混合物不一定提供足够的骨诱导作用。因此，我们在体外研究中评估了将rhBMP-2固定到琥珀酰化的I型去端胶原上对ST 2、C2 C12、MC 3 T3细胞活性的影响。此外，将固定化rhBMP-2/去端胶原和非固定化rhBMP-2/去端胶原植入Wister大鼠的皮下袋中。我们的结果表明：1）碱性磷酸酶活性证实了rhBMP-2/琥珀酰化I型端肽胶原固定在增强细胞活性方面的有效性。2)细胞内信号传导持续进行， ...更多信息 将rhBMP-2固定于琥珀酰化I型去端肽胶原时的一段时间。3)在rhBMP-2/去端胶原复合物中，成骨细胞活性明显高于未固定的rhBMP-2。为了将固定化细胞因子支架用于骨形成，我们首先检测了去端胶原蜂窝状支架对干细胞的作用。干细胞的一个例子是KUSA/A1细胞。将含有和不含去端肽胶原的KUSA/A1细胞植入SCID小鼠。单独的KUSA/A1细胞和KUSA/A1-Atelocollagen均显示出一些不透射线的区域形成，但后者显示了更大的量。在组织学上，单独的KUSA/A1细胞显示出由细胞增殖薄层包围的少量新骨形成小岛。另一方面，KUSA/Al-Atelocollagen显示了丰富的新骨形成以及细胞增殖。这一结果支持去端胶原蜂窝状支架在细胞锚定和血管侵袭中起重要作用，为新骨形成提供精确的形状和尺寸。少

项目成果

Role of O6-methylguanine-DNA methyltransferase and effect of O6-benzylguanine on the anti-tumor activity of cis-diaminedichloroplatinum(II) in oral cancer cell lines

• DOI: 10.1016/j.oraloncology.2005.05.011
• 发表时间: 2005-11-01
• 期刊: ORAL ONCOLOGY
• 影响因子: 4.8
• 作者: Maki, Y;Murakami, J;Kishi, K
• 通讯作者: Kishi, K

Cyclooxygenase 2 expression in otitis media with effusion

• DOI: 10.1016/j.amjoto.2005.07.009
• 发表时间: 2006-03-01
• 期刊: AMERICAN JOURNAL OF OTOLARYNGOLOGY
• 影响因子: 2.5
• 作者: Kakiuchi, M;Tsuiigiwa, H;Nishizaki, K
• 通讯作者: Nishizaki, K

In vivo effect of fluoride-substituted apatite on rat bone

氟化磷灰石对大鼠骨的体内作用

• 发表时间: 2005
• 期刊: Dent Mater J 24(3)
• 作者: Murakoshi;M. et al.;Tsujigiwa H;Tsujigiwa H.;Hidetsugu Tsujigiwa;Tsujigiwa H;Ohtawa Y;Rodriguez AP;Kakiuchi M;Han PP;Tsujigiwa H;Rodriguez AP;Tsujigiwa H.;Ohtawa Y.;Rodriguez AP.;Kakiuchi M.;Han PP.;Tsujigiwa H.;Rodriguez AP.;Hidetsugu Tsujigiwa;Hidetsugu Tsujigiwa;Andrea Paola Rodriguez;Yasuyuki Ohtawa;Phuu Pwint Han;Tsujigiwa Hidetsugu;Tsujigiwa H;Tsujigiwa H;Tsujigiwa H;Inoue M
• 通讯作者: Inoue M

Effects of histone deacetylase inhibitor FR901228 on the expression level of telomerase reverse transcriptase in oral cancer

• DOI: 10.1007/s00280-004-0976-x
• 发表时间: 2005-07-01
• 期刊: CANCER CHEMOTHERAPY AND PHARMACOLOGY
• 影响因子: 3
• 作者: Murakami, J;Asaumi, J;Kishi, K
• 通讯作者: Kishi, K

Efficacy of atelocollagen honeycomb scaffold in bone formation using KUSA/A1 cells

使用 KUSA/A1 细胞进行去端肽胶原蜂窝支架在骨形成中的功效

• 发表时间: 2006
• 期刊: J Biomed Mater Res A 77(4)
• 作者: Murakoshi;M. et al.;Tsujigiwa H;Tsujigiwa H.;Hidetsugu Tsujigiwa;Tsujigiwa H;Ohtawa Y;Rodriguez AP
• 通讯作者: Rodriguez AP