Differentiation induction of mesenchymal stem cells in the peripheral blood into tooth structure forming cells

诱导外周血间充质干细胞分化为牙齿结构形成细胞

• 批准号: 22791977
• 负责人: TSUJIGIWA Hidetsugu
• 金额: $ 2.5万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791977/
• 关键词: 象牙質; 骨髄; 再生; 骨髄細胞; 歯髄細胞; 象牙芽細胞; 幹細胞; GFP; 歯髄; 再生医学; 細胞外基質

Aiming the differentiation induction into odontoblasts from mesenchymal stem cells in the peripheral blood, establishment of the cells from dental pulp and bone marrow were performed. Their differentiation characterization was assessed by biochemical and histological analyses.The cells established from dental pulp expressed odontoblast specific markers, and was capable of generating dentin tissue. In the study on GFP transplantation model, bone marrow derived cells were established as a peripheral blood derived cells. The analyses of this primary culture cells revealed that stem cell like cells exist in the bone marrow, which can differentiate into tooth structure forming cells in vivo. However, some cells, which also possess pluripotency, would not be engrafted by transplantation. These data indicated the possible presence of different types of stem cells in the bone marrow. These cells are encouraging cells that possess the possibility of new innovation in the regenerative dentistry.

以从外周血中的间充质干细胞向成牙本质细胞的分化诱导为目标，进行了来自牙髓和骨髓的细胞的建立。通过生化和组织学分析评估它们的分化特征。从牙髓建立的细胞表达成牙本质细胞特异性标记物，并且能够产生牙本质组织。在GFP移植模型的研究中，建立了骨髓来源的细胞作为外周血来源的细胞。对这种原代培养细胞的分析表明，骨髓中存在干细胞样细胞，它们可以在体内分化成牙齿结构形成细胞。然而，一些也具有多能性的细胞无法通过移植来植入。这些数据表明骨髓中可能存在不同类型的干细胞。这些细胞是令人鼓舞的细胞，具有再生牙科新创新的可能性。

项目成果

Dickkopf (Dkk)-3 and β-catenin expressions increased in the transition from normal oral mucosal to oral squamous cell arcinoma

Dickkopf (Dkk)-3 和 β-catenin 表达在正常口腔粘膜向口腔鳞状细胞癌转变过程中增加

• 发表时间: 2011
• 期刊: Journal of molecular histology
• 影响因子: 3.2
• 作者: 村岡理奈,辻極秀次;中野 敬介 ,片瀬直樹,玉村 亮;富田 美穂子 ;岡藤 範正 ,長塚 仁;川 上敏行;Tsujigiwa H;Siar CH;Fujii M
• 通讯作者: Fujii M

Establishment of odontoblastic cells, which indicate odontoblast features both in vivo and in vitro

• DOI: 10.1111/jop.12080
• 发表时间: 2013-11-01
• 期刊: JOURNAL OF ORAL PATHOLOGY & MEDICINE
• 影响因子: 3.3
• 作者: Tsujigiwa, Hidetsugu;Katase, Naoki;Nagatsuka, Hitoshi
• 通讯作者: Nagatsuka, Hitoshi

Immunolocalization of Notch signaling protein molecules in a maxillary chondrosarcoma and its recurrent tumor

上颌软骨肉瘤及其复发肿瘤中Notch信号蛋白分子的免疫定位

• 发表时间: 2010
• 期刊: European Journal of Medical Research
• 影响因子: 4.2
• 作者: Siar CH;et al.
• 通讯作者: et al.

Effect of Fluoride-substituted Apatite on In Vivo Bone Formation

• DOI: 10.1177/0885328209357109
• 发表时间: 2011-05
• 期刊: Journal of Biomaterials Applications
• 影响因子: 2.9
• 作者: M. Inoue;A. Rodríguez;N. Nagai;H. Nagatsuka;R. Legeros;H. Tsujigiwa;Masahisa Inoue;E. Kishimoto;S. Takagi

Suppressive effects of 1, 4-dihydroxy-2-naphthoic acid administration on bone resorption

1, 4-二羟基-2-萘酸给药对骨吸收的抑制作用

• 发表时间: 2010
• 期刊: Osteoporos Int
• 作者: Matsubara M;Murakami J;et al
• 通讯作者: et al