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Morphological Study of Cells and Extracellular Matrices in the Vocal Fold

声带细胞和细胞外基质的形态学研究

基本信息

批准号：
17591812
负责人：
SATO Kiminori
金额：
$ 1.15万
依托单位：
Kurume University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591812/
关键词：
vocal fold larynx cells extracellular matrix hyaluronic acid glycosaminoglycan

项目摘要

Expression of CD44 (a cell surface receptor for hyaluronic acid) and the distribution of hyaluronic acid were examined in the human vocal fold mucosa.Light microscopic investigation was carried out on 10 normal larynges of newborn, infant, younger and aged adult vocal fold mucosa using Alcian Blue staining, hyaluronidase digestion study and immunohistochemistry for CD44.Prior to the appearance of hyaluronic acid in the newborn vocal fold mucosa, CD44 was expressed on the stellate cells in the macula flava and on the fibroblasts in the Reinke' s space. During infancy, hyaluronic acid appeared and was distributed in the vocal fold mucosa. Many more stellate cells in the macula flava showed CD44 expression, and a large amount of hyaluronic acid was present around the infant stellate cells, however the fibroblasts in the Reinke' s space expressed little CD44. During adulthood, hyaluronic acid was distributed in the VFM. Almost all of the SCs in the MF showed CD44 expression, and much hyaluronic acid existed around the stellate cells in the adult macula flava. However fibroblasts in the adult Reinke' s space expressed little CD44, and hyaluronic acid density in that space was lower than that in the macula flava.Stellate cells in the macula flava and CD44 co-operatively play important roles in maintaining hyaluronic acid in the human vocal fold mucosa as a vibrating tissue.

检测人声带粘膜中CD44（透明质酸的细胞表面受体）的表达和透明质酸的分布。采用阿尔新蓝染色、透明质酸酶消化研究和免疫组化对CD44进行光镜检查，对新生儿、婴儿、青少年和老年成人声带粘膜的10个正常喉进行光镜检查。 CD44在新生儿声带粘膜中表达，黄斑星状细胞和赖因克间隙成纤维细胞上表达。在婴儿期，透明质酸出现并分布在声带粘膜中。黄斑黄斑中更多的星状细胞表现出CD44表达，并且婴儿星状细胞周围存在大量透明质酸，但赖因克间隙中的成纤维细胞表达很少的CD44。成年期间，透明质酸分布在 VFM 中。 MF中几乎所有SC均表达CD44，成人黄斑黄斑星状细胞周围存在大量透明质酸。而成人Reinke间隙的成纤维细胞CD44表达量很少，并且该间隙的透明质酸密度低于黄斑。黄斑中的星状细胞和CD44协同作用，在维持人声带粘膜作为振动组织的透明质酸方面发挥着重要作用。