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Functional changes mediated by high-intraocular pressure-induced optic nerve damage in lateral geniculate body and visual cortex of monkeys.

高眼压引起猴外侧膝状体和视觉皮层视神经损伤介导的功能变化。

基本信息

批准号：
17591848
负责人：
HARA Hideaki
金额：
$ 2.24万
依托单位：
Gifu Pharmaceutical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591848/
关键词：
Glaucoma High intraocular pressure Optic nerve damage Ophthalmology Fundal change Early therapy Retinal damage PET

项目摘要

1) The purpose was to investigate time-dependent, exofocal alterations in the lateral geniculate nucleus (LGN) following NMDA-induced retinal damage in mice. Retinal damage was noted at 1 day after NMDA injection. At 7 days after NMDA injection, atrophy of the optic tract was observed on the contralateral side. In the contralateral dorsal LGN (d-LGN) and contralateral ventral LGN-lateral (vLGN-1), neurons were first decreased in number at 90 and 180 days, respectively, after NMDA injection, and neuronal cell-size was first decreased at 30 and 7 days, respectively, after NMDA injection. During this neuronal degeneration, expressions of glial fibrillary acidic protein (GFAP) and brain-derived neurotrophic factor (BDNF) were increased in the contralateral dLGN and vLGN-1, with some GFAP-positive astroglial cells also exhibiting BDNF. After NMDA injection, time-dependent, exofocal alterations occurred along the retinogeniculate pathway (from retina to LGN), and the degree of damage in LGN was region-dependent. Increased expressions of GFAP and BDNF in the affected LGN regions may play some roles in the retrograde cell-death mechanism in this nucleus.2) Alongside the IOP elevation, time-related changes in optic disc topography and RNFL thickness were demonstrated in monkey eyes using HRT and GDx. HRT (rim and cup) parameters showed good correlations with histological RNFL thickness, and significant interrelations.

1)目的是研究nmda诱导的小鼠视网膜损伤后外侧膝状核（LGN）的时间依赖性、焦外改变。注射NMDA后第1天观察到视网膜损伤。注射NMDA后第7天，观察到对侧视束萎缩。注射NMDA后，对侧LGN背侧（d-LGN）和对侧LGN腹侧-外侧（vLGN-1）神经元数量分别在90天和180天首次减少，神经元细胞大小分别在30天和7天首次减少。在神经元变性过程中，对侧dLGN和vLGN-1中胶质原纤维酸性蛋白（GFAP）和脑源性神经营养因子（BDNF）的表达增加，部分GFAP阳性星形胶质细胞也表现出BDNF。注射NMDA后，沿视网膜原状通路（从视网膜到LGN）发生时间依赖性的病灶外改变，LGN的损伤程度是区域依赖性的。受影响LGN区域GFAP和BDNF表达的增加可能在该核逆行性细胞死亡机制中起一定作用。2)利用HRT和GDx观察猴眼视盘形貌和RNFL厚度随时间的变化。HRT（边缘和杯）参数与RNFL组织学厚度有良好的相关性，且相关性显著。