Functional changes mediated by high-intraocular pressure-induced optic nerve damage in lateral geniculate body and visual cortex of monkeys.

1)目的是研究NMDA诱导的小鼠视网膜损伤后外侧膝状体核（LGN）的时间依赖性外焦改变。注射NMDA后第1天观察到视网膜损伤。注射NMDA后7天，在对侧观察到视束萎缩。在对侧背侧LGN（d-LGN）和对侧腹侧LGN-外侧（vLGN-1），神经元的数量分别在注射NMDA后90和180天首次减少，神经元的大小分别在注射NMDA后30和7天首次减少。在这种神经元变性过程中，胶质细胞酸性蛋白（GFAP）和脑源性神经营养因子（BDNF）的表达在对侧dLGN和vLGN-1中增加，一些GFAP阳性的星形胶质细胞也表现出BDNF。注射NMDA后，沿着视网膜膝状体通路（从视网膜到LGN）发生了时间依赖性的外焦改变，LGN的损伤程度具有区域依赖性。在受影响的LGN区域中GFAP和BDNF的表达增加可能在该核的逆行性细胞死亡机制中起一定作用。2）在猴眼中，使用HRT和GDx证实了随着眼压升高，视盘地形图和RNFL厚度的时间相关性变化。HRT（边缘和杯）参数与组织学RNFL厚度显示出良好的相关性，并显着相关性。

1) The purpose was to investigate time-dependent, exofocal alterations in the lateral geniculate nucleus (LGN) following NMDA-induced retinal damage in mice. Retinal damage was noted at 1 day after NMDA injection. At 7 days after NMDA injection, atrophy of the optic tract was observed on the contralateral side. In the contralateral dorsal LGN (d-LGN) and contralateral ventral LGN-lateral (vLGN-1), neurons were first decreased in number at 90 and 180 days, respectively, after NMDA injection, and neuronal cell-size was first decreased at 30 and 7 days, respectively, after NMDA injection. During this neuronal degeneration, expressions of glial fibrillary acidic protein (GFAP) and brain-derived neurotrophic factor (BDNF) were increased in the contralateral dLGN and vLGN-1, with some GFAP-positive astroglial cells also exhibiting BDNF. After NMDA injection, time-dependent, exofocal alterations occurred along the retinogeniculate pathway (from retina to LGN), and the degree of damage in LGN was region-dependent. Increased expressions of GFAP and BDNF in the affected LGN regions may play some roles in the retrograde cell-death mechanism in this nucleus.2) Alongside the IOP elevation, time-related changes in optic disc topography and RNFL thickness were demonstrated in monkey eyes using HRT and GDx. HRT (rim and cup) parameters showed good correlations with histological RNFL thickness, and significant interrelations.