The effects of transplantation of dopaminergic neuron induced from bone marrow stromal cells into amblyopia model through cerebrospinal fluid in rat

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We showed the analysis of rat deprivation amblyopia in this research. In 2005, we made the amblyopia models, differentiation of bone marrow stromal cells (BMSc) to dopaminergic neuronal cells (DNc), and cell transplantation via cerebrospinal fluid to rat amblyopia models. In 2006, we analyzed these models using antibody array and PCR. Antibody array showed up-regulation of Tyrosine hydroxylase (Th) and down-regulation of signal proteins for apoptotic and MAP kinase signaling system in amblyopic brain. PCR study showed down-regulation of Th in amblyopic retina, and down-regulation signal proteins for apoptotic and MAP kinase signaling system in both amblyopic brain and retina. In the cell transplantation group, PCR study showed up-regulation of these proteins, neurofilament and GFAP. These results suggest that the BMSc derived DNc transplantation into rat amblyopia model is effective in up-regulation of Th, anti-apoptotic pathway and the survival of neuronal cells. In the future, there is a necessity for examining the effect of cell transplantation compared with the administration of Levodopa.