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Clarification of the mechanism to cause symptoms in patients with pancreaticobiliary maljunction

阐明胰胆管连接不良患者出现症状的机制

基本信息

批准号：
17591860
负责人：
KANEKO Kenitiro
金额：
$ 1.34万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

Symptoms of choledochal cysts are caused by protein plugs. We performed proteomic analysis of protein plugs to elucidate formation mechanism. Protein plugs were obtained from three pediatric patients. with choledochal cyst. Proteins were separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Gel bands common to the samples were excised for mass spectrometry. Mass spectra were compared with the NCBI database for protein identification. Gel bands of protein plug samples were predominant at 14 kilodaltons, followed by 29 kilodaltons. Four other thin bands were common to the plug samples. Four bands (including 14 and 29 kilodaltons) were identified as lithostathine, and one band as serum albumin. Plugs consisted mostly of lithostathine, a protein secreted by pancreatic acinar cells into pancreatic juice. The mechanism involves trypsinogen and lithostathine regurgitating into the cyst through an aberrant union of pancreaticobiliary ducts. Activated trypsin cleaves soluble lithostathine into insoluble forms that aggregate to form plugs.

胆总管囊肿的症状是由蛋白质堵塞引起的。我们对蛋白质塞进行了蛋白质组学分析，以阐明形成机制。蛋白质塞取自3名儿科患者。有胆总管囊肿。用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法分离蛋白质。样品共有的凝胶条带被切除用于质谱分析。将质谱与NCBI数据库进行比较，以进行蛋白质鉴定。蛋白质塞样品的凝胶条带以14千道尔顿为主，其次是29千道尔顿。塞状样品还有另外四条共同的薄带。共鉴定出4条带(包括14K和29K)为石丹碱，1条为血清白蛋白。塞子主要由石丹碱组成，这是一种由胰腺腺泡细胞分泌到胰液中的蛋白质。其机制包括胰酶原和胆石酸通过胰胆管的异常连接反流进入囊内。激活的胰酶将可溶的石灰石分解成不溶的形式，聚集形成堵塞。

项目成果

期刊论文数量（11）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Extrahepatic portal vein morphology in children with extrahepatic portal hypertension assessed by 3-dimensional computed tomographic portography: a new etiology of extrahepatic portal hypertension

DOI：
10.1016/j.jpedsurg.2005.12.027
发表时间：
2006-04-01
期刊：
JOURNAL OF PEDIATRIC SURGERY
影响因子：
2.4
作者：
Shinohara, Tsuyoshi;Ando, Hisami;Kaneko, Kenitiro
通讯作者：
Kaneko, Kenitiro

Bile infection contributes to intrahepatic calculi formation after excision of choledochal cyst.

胆总管囊肿切除后，胆汁感染导致肝内结石形成。

DOI：
发表时间：
2005
期刊：
Pediatr Surg Int 21
影响因子：
0
作者：
T.Tainaka;K.Kaneko;K.Kaneko;T.Tainaka;T.Shinohara;T.Tainaka;Y.Ono;W.Sumida;T.Tainaka;K.Kaneko
通讯作者：
K.Kaneko

Living donor liver transplantation for congenital absence of the portal vein in a child with cardiac failure.

活体肝移植治疗心力衰竭儿童先天性门静脉缺失。