Development of new calibration procedures in Micro X-Ray Fluorescence (MXRF) using Thermal Inkjet Picofluidic System (TIPS) delivering droplets of picoliter volume, and their application for the characterization of aerosol particulate matter

使用热喷墨微微流控系统 (TIPS) 开发微 X 射线荧光 (MXRF) 的新校准程序，提供皮升体积的液滴，及其在气溶胶颗粒物表征中的应用

• 批准号: 48677760
• 负责人: Professorin Dr. Ursula Fittschen
• 金额: --
• 依托单位: Chemical Diagnostics and Engineering Group
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/48677760?language=en
• 关键词: Development; new; calibration; procedures; Micro

Total Reflection X-Ray Fluorescence (TXRF) is a powerful method for determination of traces and ultra traces of elements where sample material is limited. One major drawback of the method is that the analysis is especially sensitive for the sample morphology. This hampers the direct analysis of samples with a complex matrix (e.g. urine, blood). Commonly microliter volumes are used to deposit liquid sample material. As the control on the drying process is limited these specimens often suffer from inhomogeneous distribution of standard and analyte and easily exceed the critical thickness required for a precise analysis with TXRF (ca. 4 μm).Therefore in this study we evaluate specimen preparation of samples having a complex matrix (e.g. As in urine in a cooperation with the National Institute of Standards and Technology Gaithersburg, MD) using a prototype Thermal Inkjet Picofluidic System (TIPS) delivering picoliter volumes (1-200 pL) for direct TXRF analysis. The TIPS is exclusively available at the Los Alamos National Laboratory and has been introduced for the preparation of references in Micro X-Ray Fluorescence (MXRF) in the first part of this research stay.Essential parameters like sample morphology and chemical homogeneity, will be studied using Atomic Force Microscopy (AFM) and MXRF (focal spot 10 μm). Since the new sample preparation procedure proved its value, the TIPS will be integrated into a prototype grazing-incidence monochromatic micro XRF (GI-MMXRF) instrument. This prototype will then be evaluated for rapid and reliable analysis of various samples.

全反射X射线荧光（TXRF）是测定样品材料有限的痕量和超痕量元素的强大方法。该方法的一个主要缺点是分析对样品形态特别敏感。这妨碍了对具有复杂基质的样品（例如尿液、血液）的直接分析。通常使用微升体积来存款液体样品材料。由于对干燥过程的控制是有限的，这些样品通常遭受标准和分析物的不均匀分布，并且很容易超过TXRF精确分析所需的临界厚度（约100 μ m）。因此，在本研究中，我们使用原型热喷墨微流控系统（TIPS）评估具有复杂基质的样品的样本制备（例如，与马里兰州盖瑟斯堡的国家标准与技术研究所合作的尿液中的As），该原型热喷墨微流控系统递送皮升体积（1-200 pL）用于直接TXRF分析。TIPS是洛斯阿拉莫斯国家实验室独家提供的，在本次研究的第一部分，TIPS已被引入用于制备微X射线荧光（MXRF）的参考。将使用原子力显微镜（AFM）和MXRF（焦点10 μm）研究样品形态和化学均匀性等基本参数。由于新的样品制备程序证明了其价值，TIPS将被集成到掠入射单色显微XRF（GI-MMXRF）仪器的原型中。然后将对该原型进行评估，以快速可靠地分析各种样品。