Light microscopy for nanoscale quantum thermometry

用于纳米级量子测温的光学显微镜

• 批准号: 491245864
• 金额: --
• 依托单位: Universität Ulm
• 依托单位国家: 德国
• 项目类别: Major Instrumentation Initiatives
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/491245864?language=en
• 关键词: Light; microscopy; nanoscale; quantum; thermometry

Nanoscale measurements of temperature is crucial for understanding thermodynamics of living cells. Temperature, which is one of the most important parameters governing biochemical reactions, is usually defined for large (macroscopic) ensembles of molecules. However, approaching very small length scales, fluctuations start dominate average quantities, like temperature. Hence the use of temperature as macroscopic equilibrium thermodynamic parameter in nanoscopic intracellular compartments requires experimental and theoretical clarification. Further on important quantities like heat conductivity at nanoscale may be different from their bulk values. Yet, methods to measure temperature on the nanoscale are at early development stage. Our main goal is to establish microscopy technique able to unravel phenomena induced by ultra-local temperature gradients associated with laser heating and laser cooling. This will allow to access nanoscale heat transfer impact on the dynamics of cellular metabolism and signalling. This approach will be based on a combination of quantum sensing enabled by colour centres in diamond and advanced optical microscopy including super resolution microscopy.

纳米尺度的温度测量对于理解活细胞的热力学是至关重要的。温度是控制生物化学反应最重要的参数之一，通常用于大（宏观）分子的集合。然而，在接近非常小的长度尺度时，波动开始主导平均量，比如温度。因此，使用温度作为纳米级细胞内隔间的宏观平衡热力学参数需要实验和理论的澄清。此外，像纳米尺度的热导率这样的重要量可能与它们的体积值不同。然而，在纳米尺度上测量温度的方法还处于早期发展阶段。我们的主要目标是建立能够揭示与激光加热和激光冷却相关的超局部温度梯度引起的现象的显微技术。这将允许访问纳米尺度的热传递对细胞代谢和信号的动力学影响。这种方法将基于钻石颜色中心的量子传感和包括超分辨率显微镜在内的先进光学显微镜的结合。