Visualizing cellular ultrastructure using light microscopy in hematology

项目摘要/摘要 影像是血液学重要的诊断和研究工具。这份R21建议书，是为回应 PAR-20-140(肾脏、泌尿系统和血液系统疾病的催化工具和技术开发)，IS 旨在优化、验证和部署开创性的光学显微镜平台，用于可视化 血液学超微结构中的蛋白质。可视化他们感兴趣的特定蛋白质 超微结构(亚细胞器，~亚50 nm)目前需要相关的光学和电子显微镜 (Clem)极其复杂、耗时和昂贵，因此大多数人无法访问 血液学研究人员。PAN扩展显微镜(PanExm)是一种新的成像方法，它允许 蛋白质(和其他生物分子和细胞器)在其超微结构背景下的全细胞成像 使用传统(衍射受限)光学显微镜，具有纳米级(~10-30 nm)分辨率。有了PanExM， 固定细胞在线性维度上扩展到20倍(体积~8000倍)，同时保持亚细胞 组织、细胞蛋白质含量和抗原性。对保存下来的超微结构和蛋白质进行染色 对这些不同颜色的样品进行感兴趣的研究，并使用标准光对它们进行三维成像 显微镜可以可视化和分析原本会低于光的衍射极限的东西。 目标1专注于发展和优化扩大个人血液和骨髓的方法学。 细胞贴附或固定在载玻片上。目标2专注于优化骨髓切片的扩展，以 评估微环境中的造血细胞。我们高度协同的研究团队致力于 培训和传播血液学研究的最新技术。这一强大的新技术的应用 血液和骨髓细胞的技术将是变革性的，使血液学研究人员能够 量化感兴趣的分子在其亚细胞环境中的位置、分布和相互作用。通过 将这些高分辨率成像能力从少数几个研究小组扩展到数百个研究小组 将每个数据集的吞吐量从几周增加到几小时，PanExM将使血液学研究大众化 社区对3D结构信息的访问，并将推动在生物医学研究领域的发现 从基本的细胞生物学发现到临床病理学中的组织学应用。

Project Summary/Abstract Imaging is an important diagnostic and research tool in hematology. This R21 proposal, written in response to PAR-20-140 (Catalytic Tool and Technology Development in Kidney, Urologic, and Hematologic Diseases), is designed to optimize, validate, and deploy a groundbreaking light microscopy platform for visualization of proteins in their ultrastructural context in hematology. Visualizing specific proteins of interest in their ultrastructural (sub-organelle, ~sub-50 nm) context currently requires correlative light and electron microscopy (CLEM) which is extremely complicated, time-consuming and expensive and thereby inaccessible to most researchers in hematology. Pan Expansion Microscopy (PanExM) is a novel imaging approach that allows for imaging of proteins (and other biomolecules and organelles) in their ultrastructural context on a whole-cell basis with nanoscale (~10-30 nm) resolution using conventional (diffraction-limited) light microscopes. With PanExM, fixed cells are expanded up to 20-fold in the linear dimension (~8000 fold in volume) while maintaining subcellular organization, cellular protein content and antigenicity. Staining the preserved ultrastructure as well as the protein of interest in these samples in different colors and imaging them in three dimensions using standard light microscopy allows for visualization and analysis of what would otherwise be below the diffraction limit of light. Aim 1 is focused on developing and optimizing the methodology to expand individual blood and bone marrow cells adhered or immobilized on slides. Aim 2 is focused on optimizing expansion of bone marrow sections to assess hematopoietic cells in their microenvironment. Our highly synergistic research team is dedicated to training and dissemination of the latest technologies in hematology research. Application of this powerful new technology to blood and bone marrow cells will be transformative by enabling hematology investigators to quantify the location, distribution and interactions of molecules of interest in their sub-cellular context. By extending these high-resolution imaging capabilities from a handful to hundreds of research groups and increasing the throughput from weeks per data set to hours, PanExM will democratize the hematology research community’s access to 3D structural information and will drive discoveries across biomedical research ranging from fundamental cell biological findings to histological applications in clinical pathology.