Cell-specific analysis of spatial ion patterns of stomata under salinity in two salt sensitve crops
两种盐敏感作物盐度下气孔空间离子模式的细胞特异性分析
基本信息
- 批准号:491678431
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:德国
- 项目类别:Research Grants
- 财政年份:
- 资助国家:德国
- 起止时间:
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
The improvement of salt tolerance of crop plants represents a task of increasing relevance. Faba bean is sensitive to high salt, in particular to chloride, leading to severe growth reductions. In comparison, maize is moderately sensitive to NaCl and Cl-. We aim to analyse the interplay between tissue tolerance and stomata related processes contributing to salt tolerance under conditions of increased salt ion concentrations in leaves. Because stomatal function is a top-down regulator of many physiological processes, dysfunctions of stomata inevitably have severe detrimental effects on plant growth and development. Therefore, maintaining guard cell functionality and thereby control of the water status of plants will result in an improved performance under salinity. The stomatal complex of maize differs to that of faba bean, because monocot guard cells are surrounded by subsidiary cells, which function as a reservoir for inorganic ions. In faba bean, the cell wall, i.e. the apoplast fulfils the corresponding function. Because guard cells have poor selectivity for K+ over other monovalent cations such as Na+ and import Cl- in dependency of its concentrations in the cell walls, harmful accumulation of Na+ and Cl- ions in guard cells, might occur under conditions of NaCl exposure. This observation received only little attention to date but is potentially of tremendous significance for growth performance under stress because stomata are openings on leaf surfaces that regulate intake of CO2 for photosynthesis and water loss through transpiration. The reduction of stomatal aperture under salt induced (water) stress is an effective adjustment to salinity that must be necessarily maintained. Effects of ionic stress on guard cells are merely studied. Most studies are based on whole leaves and, thus, do not have the basis to provide new insights into guard cell stress responses and, in particular, upon effects of salt enrichment in the guard cell walls. Our recent pilot experiments on epidermal peels suggested that some of the Na+ and Cl- is taken up into guard cells under long term NaCl stress. When Na+ and Cl- are absorbed, guard cells react sensitively and can therefore have secondary effects on the entire metabolism. The accumulation of salt ions as well as the decrease in potassium, that is well known to be a side effect of Na+ accumulation, will certainly interfere with guard cells physiology because potassium is a crucial regulator for stomatal movements. Our analysis will be done on the basis of individual guard cells to increase the spatial information about the cell type-specific physiological responses. The use of the latest high-resolution methods (EDX, SIMS) enables an analysis of the ionic composition of the individual guard cells.
提高农作物的耐盐性是一项日益重要的任务。蚕豆对高盐特别是氯化物敏感,导致生长严重下降。相比之下,玉米对 NaCl 和 Cl- 中等敏感。我们的目的是分析组织耐受性和气孔相关过程之间的相互作用,这些过程在叶片盐离子浓度增加的条件下有助于耐盐性。由于气孔功能是许多生理过程的自上而下的调节者,气孔功能障碍不可避免地对植物的生长发育产生严重的不利影响。因此,维持保卫细胞功能并由此控制植物的水分状况将改善盐度下的性能。玉米的气孔复合体与蚕豆的气孔复合体不同,因为单子叶植物保卫细胞被辅助细胞包围,辅助细胞充当无机离子的储存库。在蚕豆中,细胞壁,即质外体,履行相应的功能。由于保卫细胞对 K+ 的选择性比其他单价阳离子(例如 Na+)差,并且输入 Cl-(取决于其在细胞壁中的浓度),因此在 NaCl 暴露的条件下,保卫细胞中可能会发生 Na+ 和 Cl- 离子的有害积累。迄今为止,这一观察结果很少受到关注,但对于胁迫下的生长性能可能具有巨大意义,因为气孔是叶子表面的开口,可以调节光合作用的二氧化碳摄入量和蒸腾作用的水分流失。在盐诱导(水)胁迫下气孔孔径的减小是对盐度的有效调节,必须保持这一点。仅研究了离子应激对保卫细胞的影响。大多数研究都是基于整个叶子,因此没有基础来提供有关保卫细胞应激反应的新见解,特别是保卫细胞壁中盐富集的影响。我们最近对表皮进行的初步实验表明,在长期 NaCl 胁迫下,一些 Na+ 和 Cl- 被吸收到保卫细胞中。当 Na+ 和 Cl- 被吸收时,保卫细胞反应灵敏,因此会对整个新陈代谢产生二次影响。盐离子的积累以及钾的减少(众所周知,这是钠积累的副作用)肯定会干扰保卫细胞的生理机能,因为钾是气孔运动的关键调节剂。我们的分析将在单个保卫细胞的基础上进行,以增加有关细胞类型特异性生理反应的空间信息。使用最新的高分辨率方法(EDX、SIMS)可以分析各个保护单元的离子组成。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Professor Dr. Christian Zörb其他文献
Professor Dr. Christian Zörb的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Professor Dr. Christian Zörb', 18)}}的其他基金
Unraveling salt stress effects in guard cells of salt stressed Vicia faba L genotypes that contrast in their degree to salt tolerance
揭示盐胁迫对蚕豆 L 基因型保卫细胞的影响,其程度与耐盐性形成鲜明对比
- 批准号:
320167376 - 财政年份:2016
- 资助金额:
-- - 项目类别:
Research Grants
Proteomanalytische Untersuchung der Expansin-vermittelten Wachstumsdepression zweier unterschiedlich salzresistenter Maishybriden
两种不同耐盐玉米杂交种扩展蛋白介导的生长抑制的蛋白质分析研究
- 批准号:
179592771 - 财政年份:2010
- 资助金额:
-- - 项目类别:
Research Grants
Erstellung eines salzresistenten Maishybriden: Kombination von Na+-Exklusion und osmotischer Anpassung und deren biochemische Analyse
抗盐玉米杂交种的创建:钠排除和渗透调节的结合及其生化分析
- 批准号:
5312062 - 财政年份:2001
- 资助金额:
-- - 项目类别:
Research Grants
相似国自然基金
新生儿坏死性小肠结肠炎中去泛素化酶USP15调控ILC3分化损伤肠道粘膜屏障的致病机制研究
- 批准号:82371711
- 批准年份:2023
- 资助金额:49.00 万元
- 项目类别:面上项目
花胶鱼类物种Species-specific PCR和Multiplex PCR鉴定体系研究
- 批准号:31902373
- 批准年份:2019
- 资助金额:23.0 万元
- 项目类别:青年科学基金项目
Dravet综合征基因突变分析及突变来源研究
- 批准号:81171221
- 批准年份:2011
- 资助金额:58.0 万元
- 项目类别:面上项目
睾丸特异性新基因TSC29的表达调控机制及其功能研究
- 批准号:81170613
- 批准年份:2011
- 资助金额:54.0 万元
- 项目类别:面上项目
RNA结合蛋白CUG-BP1对于mRNA降解的调控机制研究
- 批准号:31000570
- 批准年份:2010
- 资助金额:20.0 万元
- 项目类别:青年科学基金项目
新生隐球菌减数分裂特异性基因ISC10的生理功能研究
- 批准号:30970130
- 批准年份:2009
- 资助金额:30.0 万元
- 项目类别:面上项目
寻找精神分裂症的调节性遗传变异
- 批准号:30870899
- 批准年份:2008
- 资助金额:45.0 万元
- 项目类别:面上项目
相似海外基金
CAREER: Cell Specific Analysis of Conserved Spindle Orientation Factors
职业:保守纺锤体方向因素的细胞特异性分析
- 批准号:
2409495 - 财政年份:2024
- 资助金额:
-- - 项目类别:
Continuing Grant
Determining the effect of early resource scarcity on adolescent addiction-related behavior and cell-type specific transcription
确定早期资源稀缺对青少年成瘾相关行为和细胞类型特异性转录的影响
- 批准号:
10825012 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Gene regulatory network control of olfactory cortex cell type specification
嗅觉皮层细胞类型规范的基因调控网络控制
- 批准号:
10656692 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Bridging the gap: joint modeling of single-cell 1D and 3D genomics
弥合差距:单细胞 1D 和 3D 基因组学联合建模
- 批准号:
10572539 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Genetic analysis of the Robo3+ glycinergic amacrine cell
Robo3 甘氨酸无长突细胞的遗传分析
- 批准号:
10749795 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Single-cell Transcriptomic Analysis of Cell Type Plasticity in Barrel Cortex of Normal and Autism Model Mice
正常和自闭症模型小鼠桶状皮层细胞类型可塑性的单细胞转录组分析
- 批准号:
10750812 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Integrated fragment-based phenotypic screening and chemoproteomics for identification of novel small cell lung cancer-specific targets
基于片段的表型筛选和化学蛋白质组学相结合,用于鉴定新型小细胞肺癌特异性靶标
- 批准号:
10577507 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Molecular Control of Plasmacytoid Dendritic Cell Development and Function
浆细胞样树突状细胞发育和功能的分子控制
- 批准号:
10583989 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Cell Type-Specific Analysis of Immune Checkpoint Signalling Networks Underpinning Cancer Immunotherapy
支持癌症免疫治疗的免疫检查点信号网络的细胞类型特异性分析
- 批准号:
MR/W025507/1 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Research Grant