Interaction of a plant branching architecture affecting fungal effector with the plant E3 ubiquitin ligases RGLG1 and RGLG2

影响真菌效应子的植物分支结构与植物 E3 泛素连接酶 RGLG1 和 RGLG2 的相互作用

• 批准号: 493870324
• 负责人: Professor Dr. Jan Schirawski
• 金额: --
• 依托单位: Lehrstuhl für Genetik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/493870324?language=en
• 关键词: Interaction; plant; branching; architecture; affecting

Biotrophic fungal plant pathogens interact with their host plants through the secretion of effectors, small secreted proteins that end up within the host cell tissues and impact plant gene expression or regulation of plant protein activity. A few effectors have been shown to alter plant development possibly for better fungal colonization success. The effector SAD1 of Sporisorium reilianum has been shown to alter plant inflorescence branching architecture both in S. reilianum-infected maize (Zea mays cv. ‘Gaspe Flint’) and in Arabidopsis thaliana lines stably expressing SAD1, by an as yet unknown mechanism. We have shown that SAD1 interacts with the E3 ubiquitin ligases RGLG1 and RGLG2 of both maize and Arabidopsis. In this proposal, we want to characterize the functional interaction of SAD1 with these E3 Ubiquitin ligases, in vitro and in planta, its localization and distribution within the plant tissue, as well as the putative role of myristoylation of both RGLG1/2 and SAD1 for interaction and distribution within subcellular compartments. This will lead to a better understanding of how effectors may impact primary plant developmental processes, which in turn may also lead to a better understanding of the factors determining plant inflorescence branching architecture.

活体营养型真菌植物病原体通过分泌效应物与其宿主植物相互作用，效应物是最终在宿主细胞组织内并影响植物基因表达或植物蛋白活性调节的小分泌蛋白。一些效应子已被证明可以改变植物发育，可能是为了更好的真菌定殖成功。玉米孢霉的效应子SAD 1已被证明可以改变植物花序分枝结构，无论是在S.感病玉米（Zea mays cv.“加斯佩弗林特”）和稳定表达SAD 1的拟南芥品系中，通过一种至今未知的机制。我们已经证明SAD 1与玉米和拟南芥的E3泛素连接酶RGLG 1和RGLG 2相互作用。在这个建议中，我们要表征SAD 1与这些E3泛素连接酶的功能相互作用，在体外和在植物中，其在植物组织内的定位和分布，以及RGLG 1/2和SAD 1的肉豆蔻酰化在亚细胞区室内的相互作用和分布的假定作用。这将导致更好地理解效应器如何影响初级植物发育过程，这反过来也可能导致更好地理解决定植物花序分枝结构的因素。