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Search for gcncs rcgulating acuronal plasticity

搜索 gcncs 调节轴突可塑性

基本信息

批准号：
07278105
负责人：
NODA Makoto
金额：
$ 132.67万
依托单位：
The University of Kyoto
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1998
项目状态：
已结题

项目摘要

Noda et al. analyzed the mouse "Nedd" genes cloned based on their abundant expression in early embryonic brain and down-regulation in adult brain. The findings include transient up-regulation of Nedd2/caspase-2 gene after brain ischemia. requirement of Nedd5, a novel cytoskeletal protein with GTPase activity, in cytokinesis. and its accumulation in the neurotibrillary tangles in Alzheimer's disease. Using single cell-RT-PCR/differenlial display, Yamamori et al, discovered a novel rat-specific repetitive sequence (Bsr) as a gene up-regulated in cultured cerebellum Purkinje cells after treatment with AMPA plus cOMP analog. Sato and Kamino et al, discovered a novel, GABA blocker-insensitive GABA receptor (GABAD) in chick embryo brain stem which provoke depolarizing response and intracellular Ca2+ accumulation. Hirano et al, demonstrated, by using knockout mice, that δ2 glutamate receptor subunit is involved in LTD and motor Icarning. Using culturc ccrcbcllum cclls, they also demonstratod … More the involvcmcnt of MAP kinase in LTD and furlher developed an cxperi mental systcm that can be used to monitor LTD over several days. Tomooka et al, established and characterived a series of cell lines capable of differentiating into various types of neurons and glias from ccrcbcllum of a mice lacking p53 gene. Fukui ct al, dcvelopcd a system to induce head morphogenesis in Xenopus animal cap (prospective ectoderm) in vitro. Usino this system, they cloned a series of genes induced during brain development and analyzed their expression patterns in vivo by whole-mount in situ hybridization. Nose et al. isolated several genes expressed in specific muscle and the attached neurons by enhancer trap screening in Drosophila. Their products include a cell surface protein (Capricious), a secreted protein (M-spondin), and a transcription factor (Msh), and their importance during neuro-muscular development has been demonstrated by ectopic expression. Ishihara et al, identified the target of the nematode hch-1 mutation, which shows delayed hatching and abnormal neuroblast migration, as a gene encoding a metalloproteinase of the Tolloid/BMP-1 family. Less

Noda等人分析了克隆的小鼠“Nedd”基因，基于其在早期胚胎脑中的丰富表达和在成年脑中的下调。研究结果包括脑缺血后Nedd 2/caspase-2基因的瞬时上调。需要Nedd 5，一种新的细胞骨架蛋白，具有GT3活性，在胞质分裂。以及它在阿尔茨海默氏病的神经纤维缠结中的积累。Yamamori等利用单细胞RT-PCR/差异显示技术发现了一种新的大鼠特异性重复序列（Bsr），该基因在培养的小脑浦肯野细胞中被AMPA + cOMP类似物处理后上调。Sato和Kamino等在鸡胚脑干中发现了一种新的GABA受体（GABAD），它对GABA受体阻断剂不敏感，能引起去极化反应和细胞内Ca ~（2+）的积累。Hirano等人通过使用基因敲除小鼠证明，δ2谷氨酸受体亚基参与LTD和运动Icarning。使用culturc ccrcblum cclls，他们还证明了 ...更多信息 LTD中MAP激酶的参与和Furlher开发了一种可用于数天监测LTD的实验系统。Tomooka等从缺乏p53基因的小鼠脑细胞中建立了一系列能分化成各种类型神经元和胶质细胞的细胞系，并对其进行了鉴定。福井等在体外建立了一个诱导非洲爪蟾动物帽（预期的外胚层）头部形态发生的系统。利用这个系统，他们克隆了一系列在大脑发育过程中诱导的基因，并通过整体原位杂交分析了它们在体内的表达模式。Nose等人通过增强子捕获筛选，分离出了果蝇中在特定肌肉和附着神经元中表达的几个基因。它们的产物包括细胞表面蛋白（Capricious）、分泌蛋白（M-spondin）和转录因子（Msh），它们在神经肌肉发育过程中的重要性已通过异位表达得到证实。Ishihara等鉴定了线虫hch-1突变的靶点，其显示出延迟孵化和异常成神经细胞迁移，作为编码Tolloid/BMP-1家族的金属蛋白酶的基因。少