Search for gcncs rcgulating acuronal plasticity

搜索 gcncs 调节轴突可塑性

基本信息

批准号：
07278105
负责人：
NODA Makoto
金额：
$ 132.67万
依托单位：
The University of Kyoto
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1998
项目状态：
已结题

项目摘要

Noda et al. analyzed the mouse "Nedd" genes cloned based on their abundant expression in early embryonic brain and down-regulation in adult brain. The findings include transient up-regulation of Nedd2/caspase-2 gene after brain ischemia. requirement of Nedd5, a novel cytoskeletal protein with GTPase activity, in cytokinesis. and its accumulation in the neurotibrillary tangles in Alzheimer's disease. Using single cell-RT-PCR/differenlial display, Yamamori et al, discovered a novel rat-specific repetitive sequence (Bsr) as a gene up-regulated in cultured cerebellum Purkinje cells after treatment with AMPA plus cOMP analog. Sato and Kamino et al, discovered a novel, GABA blocker-insensitive GABA receptor (GABAD) in chick embryo brain stem which provoke depolarizing response and intracellular Ca2+ accumulation. Hirano et al, demonstrated, by using knockout mice, that δ2 glutamate receptor subunit is involved in LTD and motor Icarning. Using culturc ccrcbcllum cclls, they also demonstratod … More the involvcmcnt of MAP kinase in LTD and furlher developed an cxperi mental systcm that can be used to monitor LTD over several days. Tomooka et al, established and characterived a series of cell lines capable of differentiating into various types of neurons and glias from ccrcbcllum of a mice lacking p53 gene. Fukui ct al, dcvelopcd a system to induce head morphogenesis in Xenopus animal cap (prospective ectoderm) in vitro. Usino this system, they cloned a series of genes induced during brain development and analyzed their expression patterns in vivo by whole-mount in situ hybridization. Nose et al. isolated several genes expressed in specific muscle and the attached neurons by enhancer trap screening in Drosophila. Their products include a cell surface protein (Capricious), a secreted protein (M-spondin), and a transcription factor (Msh), and their importance during neuro-muscular development has been demonstrated by ectopic expression. Ishihara et al, identified the target of the nematode hch-1 mutation, which shows delayed hatching and abnormal neuroblast migration, as a gene encoding a metalloproteinase of the Tolloid/BMP-1 family. Less

Noda等。分析了小鼠“ NEDD”基因基于早期胚胎大脑中的丰富表达和成人大脑下调的基因。研究结果包括脑缺血后NEDD2/caspase-2基因的短暂上调。 NEDD5的需求是一种新型的细胞骨架蛋白，具有GTPase活性，在细胞因子中。以及它在阿尔茨海默氏病的神经纤维缠结中的积累。 Yamamori等人使用单细胞-RT-PCR/分离显示，发现了一种新型的大鼠特异性重复序列（BSR），作为一种基因，在用AMPA加上Comp Analog处理后，在培养的小脑Purkinje细胞中上调。 Sato和Kamino等人在雏鸡胚胎脑干中发现了一种新型的GABA阻滞剂不敏感的GABA受体（GABAD），这会引起分裂反应和细胞内Ca2+积累。 Hirano等人通过使用基因敲除小鼠证明了Δ2谷氨酸受体亚基参与LTD和运动型。使用培养的CCRCBCLUM CCLLS，它们还证明了……更多的是Ltd中MAP激酶的参与CMCNT和Furlher开发了一种CXPERI心理系统，可在几天内用于监测LTD。 Tomooka等人建立并表征了一系列能够从缺乏p53基因的小鼠的CCRCBCLUM区分为各种类型的神经元的细胞系。 Fukui CT AL，DCEVEMENCD在体外诱导爪蟾动物帽（前瞻性外胚层）的头部形态发生的系统。 USINO这个系统，他们克隆了一系列在大脑发育过程中诱导的基因，并通过原位原位杂交分析了它们在体内的表达模式。鼻子等。通过在果蝇中的增强子陷阱筛选中分离出在特定肌肉和附着的神经元中表达的几个基因。他们的产品包括细胞表面蛋白（反复无常），分泌的蛋白质（M链霉素）和转录因子（MSH），并且通过依托型表达证明了它们在神经肌肉发育过程中的重要性。 Ishihara等人确定了线虫HCH-1突变的靶标，该靶标显示了延迟的孵化和神经细胞迁移异常，它是编码Tolloid/BMP-1家族的金属蛋白酶的基因。较少的