Roles and Regulatory Mechanisms of Extracellular Matrix Remodeling in vivo

体内细胞外基质重塑的作用和调控机制

• 批准号: 16GS0311
• 负责人: NODA Makoto
• 金额: $ 385.8万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Creative Scientific Research
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16GS0311/
• 关键词: がん; 発生; マウス遺伝学; MMP; ADAM; Notch; シグナル伝達; RECK; 細胞マトリックス; fibronectin; 細胞移動; 細胞密度; 血清濃度; 酸素濃度; 条件的遺伝子欠損マウス; Ras; 発現遺伝子プロファイリング; 軟骨分化; MT1-MMP; CD13; Rg13; profilin II; MMP-2; Sept4; MRF; 筋肉発生; Rb; N-Ras; 甲状腺腫瘍; septin; 精子形成 /

RECK, first isolated as a transformation suppressor gene, encodes a membrane-anchored glycoprotein essential for mouse embryogenesis and down-regulated in various type of human cancer. Our studies at the molecular, cellular, and whole animal levels revealed that RECK forms cowbell-shaped dimer, regulates a wide spectrum of extracellular metalloproteases, and protects cell surface molecules as well as extracellular matrix components. We also found that RECK expression is under dynamic regulations by various external stimuli, including growth factors, cell density, and oxygen concentration.

RECK基因是一种细胞转化抑制基因，编码一种膜锚定糖蛋白，在小鼠胚胎发育过程中起重要作用，在多种人类肿瘤中表达下调。我们在分子、细胞和整个动物水平的研究表明，RECK形成牛铃状二聚体，调节广谱的细胞外金属蛋白酶，并保护细胞表面分子以及细胞外基质成分。我们还发现RECK的表达受到各种外部刺激的动态调节，包括生长因子、细胞密度和氧浓度。

项目成果

Reek forms cowbell-shaped dimers and inhibits matrix metallopro teinase-catalyzed cleavage of fibronectin

Reek 形成牛铃形二聚体并抑制基质金属蛋白酶催化的纤连蛋白裂解

• 发表时间: 2009
• 期刊: J Biol Chem 284
• 作者: Hatta M;Matsuzaki T;Morioka Y;Yoshida Y;Noda M.;Hatta M;Morioka Y;Omura A
• 通讯作者: Omura A

The membrane-anchored metalloproteinase regulator RECK stabilizes focal adhesions and anterior-posterior polarity in fibroblasts

• DOI: 10.1038/onc.2008.486
• 发表时间: 2009-03-01
• 期刊: ONCOGENE
• 影响因子: 8
• 作者: Morioka, Y.;Monypenny, J.;Noda, M.
• 通讯作者: Noda, M.

Localization of the membrane-anchored MMP-regulator RECK at the neuromuscular junctions

• DOI: 10.1111/j.1471-4159.2007.04977.x
• 发表时间: 2008-01-01
• 期刊: JOURNAL OF NEUROCHEMISTRY
• 影响因子: 4.7
• 作者: Kawashima, Satoshi;Imamura, Yukio;Noda, Makoto
• 通讯作者: Noda, Makoto

The reversion-inducing cysteine-rich protein with Kazal motifs (RECK) interacts with membrane type 1 matrix metalloproteinase and CD13/aminopeptidase n and modulates their endocytic pathways

• DOI: 10.1074/jbc.m610948200
• 发表时间: 2007-04-20
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Miki, Takao;Takegami, Yujiro;Takahashi, Chiaki
• 通讯作者: Takahashi, Chiaki

RECK Forms Cowbell-shaped Dimers and Inhibits Matrix Metalloproteinase-catalyzed Cleavage of Fibronectin

• DOI: 10.1074/jbc.m806212200
• 发表时间: 2009-02-06
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Omura, Akira;Matsuzaki, Tomoko;Noda, Makoto
• 通讯作者: Noda, Makoto