Elucidating the role of extracellular matrix remodeling on the fate and behavior of neuron

阐明细胞外基质重塑对神经元命运和行为的作用

• 批准号: 17024026
• 负责人: NODA Makoto
• 金额: $ 11.52万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17024026/
• 关键词: がん; 発生; マウス遺伝学; MMP; ADAM; Notch; シグナル伝達; RECK; 細胞外マトリックス; fibronectin; 細胞密度; 細胞移動; 酸素濃度; 神経筋接合部; 骨格筋発生; MMP7; focal adhesion; 細胞運動; 神経分化; nestin; 白質病変; MMP-2; パーキンソン病; Sept4; MRF; 筋肉発生; Rb; N-ras

RECK, first identified as a transformation suppressor gene, is expressed at relatively high levels in neural precursor cells (NPCs) in mouse embryos; in Reck-deficient mice, NPCs show reduced proliferation and precocious differentiation. Our study to understand the mechanism of this phenomenon revealed that Reck inhibits ADAM10- mediated Notch-ligand shedding. We also found that in the diaphragm of late-stage embryos, Reck rapidly accumulates in the neuromuscular junctions, mainly in the secondary folds of the post-synaptic muscle membrane.

RECK，首先被确定为转化抑制基因，在小鼠胚胎的神经前体细胞（NPC）中以相对高的水平表达;在Reck缺陷小鼠中，NPC表现出增殖减少和早熟分化。我们的研究，以了解这种现象的机制表明，Reck抑制ADAM 10介导的Notch配体脱落。我们还发现，在晚期胚胎的隔膜中，Reck迅速积聚在神经肌肉接头中，主要是在突触后肌肉膜的次级褶皱中。

项目成果

The membrane-anchored metalloproteinase regulator RECK stabilizes focal adhesions and anterior-posterior polarity in fibroblasts

• DOI: 10.1038/onc.2008.486
• 发表时间: 2009-03-01
• 期刊: ONCOGENE
• 影响因子: 8
• 作者: Morioka, Y.;Monypenny, J.;Noda, M.
• 通讯作者: Noda, M.

Density-and serum-dependent regulation of the neck tumor suppressor in mouse embryo fibroblasts.

小鼠胚胎成纤维细胞中颈部肿瘤抑制因子的密度和血清依赖性调节。

• 发表时间: 2009
• 期刊: Cell Signal 21
• 作者: Hatta M;Matsuzaki T;Morioka Y;Yoshida Y;Noda M..;Hatta M
• 通讯作者: Hatta M

The membrane-anchored MMP-regulator RECK is a target of myogenic regulatory factors

• DOI: 10.1038/sj.onc.1208733
• 发表时间: 2005-09-01
• 期刊: ONCOGENE
• 影响因子: 8
• 作者: Echizenya, M;Kondo, S;Noda, M
• 通讯作者: Noda, M

Localization of the membrane-anchored MMP-regulator RECK at the neuromuscular junctions

• DOI: 10.1111/j.1471-4159.2007.04977.x
• 发表时间: 2008-01-01
• 期刊: JOURNAL OF NEUROCHEMISTRY
• 影响因子: 4.7
• 作者: Kawashima, Satoshi;Imamura, Yukio;Noda, Makoto
• 通讯作者: Noda, Makoto

The reversion-inducing cysteine-rich protein with Kazal motifs (RECK) interacts with membrane type 1 matrix metalloproteinase and CD13/aminopeptidase n and modulates their endocytic pathways

• DOI: 10.1074/jbc.m610948200
• 发表时间: 2007-04-20
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Miki, Takao;Takegami, Yujiro;Takahashi, Chiaki
• 通讯作者: Takahashi, Chiaki