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発達脳における可塑的シナプス伝達の量子解析

发育中大脑中可塑性突触传递的量子分析

基本信息

批准号：
08271223
负责人：
小田洋一
金额：
$ 1.28万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
1996
资助国家：
日本
起止时间：
1996 至无数据
项目状态：
已结题

项目摘要

脳神経回路の形成におけるシナプス伝達機能と脳機能の発達を明らかにすることを目的とし,軟骨魚の最大の網様体脊髄路ニューロンであるマウスナ-(M-)細胞を用いて主に電気生理学的なアプローチで研究を行った.M-細胞は魚類や幼生の両生類の延髄に左右一対存在し,侵害刺激からの逃避反応をトリガーする司令ニューロンであり,そのシナプス機能の発達は運動機能の発達に直接結びつく.ゼブラフィッシュのM-細胞は分化・シナプス形成過程に関する形態学的知見から,発達期のシナプスを研究するのに適した系である.しかし,シナプスの構成や機能に関する知見は極めて乏しい.そこで,成魚のM-細胞の入出力回路を明らかにすることから始めた.M-細胞特有のグリア構造であるaxon capによる電場電位を指標にM-細胞に微小電極を刺入すると,脊髄刺激によって軸索側枝からの反回性抑制が記録された.また,聴覚刺激を与えると,M-細胞の樹状突起上のgap junctionとグルタミン酸作働性シナプスを介する急峻な脱分極電位とそれに続く抑制性シナプス応答が記録された.反回性および順行性抑制性シナプス応答はグリシン受容体の阻害剤であるストリキニンで消失した.したがって,M-細胞の聴覚入力と出力信号はグリシン作動性のfeedforward抑制とfeedback抑制によって強力に制御されていることが示唆された.シナプス形成期の抑制性シナプスの伝達特性を調べるために,単位シナプス電位(unitary IPSP)の振幅のゆらぎを量子解析するシステムを確立した.M-細胞に単シナプス結合する抑制性介在ニューロンの軸索に微小電極を刺入し単一ニューロンを賦活しunitaryIPSPを記録した.そのゆらぎは量子的に振る舞い,二項分布を仮定した最尤推定から伝達物質の放出確率(p),量子サイズ(q),放出部位数(N)を推定した.聴神経のテタヌス刺激で発現する長期増強では,p(およびN)が上昇し,qは一定であった.このような伝達特性を持つ抑制性シナプスが,発達初期において逃避運動の獲得・発達や適応に果たす役割を,今後探る.

The network is designed to achieve the goal of achieving the goal of the largest network. (M -) computers are used in the field of computer physiology in the field of computer physiology. M-cell is used in the field of computer physiology. M-cell is used to improve the performance of early childhood biology. Infringing stimuli to evade counter-attacks the commander of the anti-terrorist units does not have to do so, and the equipment is capable of achieving that the mobile machines can reach the level of direct delivery. There are many problems in the process of cell differentiation, such as the formation of knowledge about the morphology of the disease, and the development of research in the process. The machine will be able to tell you that there is no need for you. The input and output circuit of the M-cell indicates that there is a significant difference between the input and output circuit. The M-cell is unique to the axon cap. The electrical potential of the M-cell indicates that the micro-electric pole of the M-cell is pierced into the circuit, and the spinal stimulation has a negative inhibitory effect on the response. In this case, there is no significant difference between the stimulation and the stimulation of the gap junction, the stimulation and the inhibition of the growth of the M-cell. in this paper, we report that there is an increase in the number of cells in the brain. Reverse sexual inhibition, sexual inhibition, sex, sex, sex and sex. The signal of the input force is the output signal of the input force. The action is the feedforward suppression, the feedback suppression, the strength control and the control. During the formation period of the device, the characteristics of the inhibitive device are sensitive to the signal, and the location of the unitaryIPSP is the amplitude of the signal. The quantum analysis of the quantum analysis device makes sure that the switch is on. M-cell cycle is combined with the effect of the inhibitory effect on the temperature field. The microelectronic device plays an important role in the monitoring of the unitaryIPSP records. The number of locations (N), the number of sites (N), and the number of sites (N). As a result, there will be a long-term increase in the number of people who will be able to increase their strength for a long time, and there will be a lot of trouble in the first place. In the early days, when it was possible to evade activities, it was possible to achieve the results of service cutting, and we will explore it later.