Bio-organic Chemical Studies on the Floral Scent Formation and Flower Induction

1.大马士革玫瑰（Rosa damascena， R.）花的日花香释放。在大马士革玫瑰（Rosa damascena quatra saison）和玫瑰（R.）中观察到2-苯乙醇(1)释放的节律周期。“Hoh-Jun”，虽然R.damascena Mill。瞬态释放1.2。在β-葡萄糖苷酶抑制剂开始饲喂后的白天，饲喂组2-苯乙基β- d -葡萄糖苷(2)的含量基本保持不变，而对照组在开始饲喂后含量有所下降，但饲喂组在展开过程中含量基本保持不变，从大马士革玫瑰花瓣中获得了1个β-葡萄糖苷酶的克隆。虽然它的功能还不清楚。1和2 [^2H_8]-1和[^2H_8]-2必须由[^2H_8] l -苯丙氨酸生物合成，[^2H_8] l -苯丙氨酸α-位置保留氘原子。我们将氘标记的中间体放入R中。“Hoh-Jun”花。证实了苯乙醛肟的高掺入。我们提出了1和2.4的合理的生物合成途径。参与花香化合物生物合成的o -甲基转移酶的克隆。从花瓣中分离到新的OMT POMT，并利用MALDI-TOFMS对其部分序列进行了表征。我们还证实了玫瑰花中存在类胡萝卜素裂解酶。KODA，是一种参与黄萎黄花诱导的化合物。通过LC-SIM和LC-MS/MS分析，首次在花诱导条件下（16 h暗暴露）培养的Pharbitis幼苗中鉴定出紫色α-酮醇亚麻酸（KODA）。用LC-MS-MS和NMR对KODA的一种代谢产物进行了表征。

1.Diurnal Floral Scent Emission form flowrs of Rosa damascena, R.'Hoh-Jun' Rhythmic cycles in the emission of 2-phenyletanol (1) are observed in Rosa damascena quatra saison and R.'Hoh-Jun', although R.damascena Mill.transiently emits 1.2.Role of glucosidase inhibitor in 2-phenylethanol emission During the day-time after the feeding of β-glucosidase-inhibitor commenced, the level of 2-phenylethyl β-D-glucopyranoside (2) remained fairly constant in the fed group, but decreased in feeding commenced in the control group, but the concentration was almost constant during the unfurling process in the fed group One β-glucosidase clone was obtained from the petals of Rose damascena Mill.although its function is not clarified yet.3.Biogenesis of 1 and 2 [^2H_8]-1 and [^2H_8]-2 must be biosynthesized from [^2H_8] L-phenylalanine with a retention of the deuterium atom at α-position of [^2H_8] L-phenylalanine.We fed deuterium-labeled intermediates into R.'Hoh-Jun' flowers.A high incorporation of phenylacetaldoxime was confirmed.We have proposed a plausible biosynthetic pathway for 1 and 2.4.Cloning of O-methyltransferases involved in the biosynthesis of floral scent compounds. New OMT, POMT, was purified from flower petals, and characterized the partial sequences by MALDI-TOFMS.We also confirmed the presence of carotenoid cleavage enzymes in rose flowers.5.KODA, a compound involved in flower-induction of Pharbitis nil.Violet (collaborative reseearch with Dr.Yokoyama, Shiseido Co. Ltd) α-Ketol linolenic acid (KODA) was identified for the first time in seedlings of Pharbitis grown under a flower-inductive condition (16-h dark exposure), by means of LC-SIM and LC-MS/MS analyses.One of the metabolites of KODA has been characterized by LC-MS-MS and NMR.