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Biosyntheis of volatile C-13 norisoprenoid

挥发性 C-13 去甲异戊二烯的生物合成

基本信息

批准号：
13460047
负责人：
WATANABE Naoharu
金额：
$ 10.24万
依托单位：
Shizuoka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13460047/
关键词：
C-13 norisoprenoid aroma precursors damascenone Rosa damascena Mill.star fruit quince fruit green tea leaves carotenoid cleavage enzymes C13ノルソプレノイド Rosa damascena Mill C13-ノルイソプレノイドバラ茶葉 star fruit

项目摘要

1.Damascenone progenitors in the flowers of Rosa damascena Mill.Two damascenone progenitors have been isolated from the flowers of R. damascena Mill. Their chemical structures were determined to be megastigma-6,7-dien-3,5,9-triol 9-Ο-β-D-glucolyranosides. The stereo chemistry for each compound was also determined by the direct comparison of their NMR spectra as well as chromatographic profiles on HIPLC.2.Damascenone progenitors in green te extractsDuring the course of sterilization of green tea extract for production of canned or bottled green tea drinks, damascenone was generated as off-flavor compound. The progenitors were isolated and identified to be acetylenic diol and allenic triol glucosides. Their stereo chemistries were also determined by comparison with synthetic specimens. We have also proposed the conversion routes for these compounds to damascenone.3.Carotenoid cleavage enzymes being involved in the formation of C13-norisoprenoidsC-13 Norisoprenoid volatile compounds are speculated to be generated from carotenoids such as β-caroten, neoxantin by the action of carotenoid cleavage enzymes in plant tissues, such as some fruits and flowers. We have, for the first time, characterized an enzyme from star fruit (Averrhoa carambola) and green tea leaves. Similar enzymes were characterized from animals and quince fruits by the group of Prof. Winterhlater. We have characterized not only the biochemical profiles, but also the products on the enzymatic reaction from b-carotene.

1.从玫瑰花中分离到两个大马酮类祖细胞。卡拉米尔。经鉴定，其化学结构为大Stigma-6，7-dien-3，5，9-triol 9-O-β-D-glucolyranosides。每种化合物的立体化学也通过直接比较它们的NMR光谱以及HPLC上的色谱图来确定。2.绿色茶提取物中的大马士革酮前体在用于生产罐装或瓶装绿色茶饮料的绿色茶提取物的灭菌过程中，产生了作为异味化合物的大马士革酮。分离得到的祖细胞经鉴定为炔二醇和丙三醇糖苷。它们的立体化学也通过与合成样品的比较来确定。3.类胡萝卜素裂解酶参与C-13-去甲异戊二烯类化合物的形成C-13-去甲异戊二烯类挥发性化合物推测是由植物组织中的类胡萝卜素裂解酶作用产生的。我们首次从星星果实（杨桃）和绿色茶叶中提取了一种酶。Winterhlater教授的研究小组从动物和温柏果实中鉴定出类似的酶。我们的特点不仅是生化档案，但也从b-胡萝卜素的酶促反应的产物。