Anesthetic Substances and Other Key Compounds Related to Biologically Intriguing Phenomena

麻醉物质和其他与有趣的生物学现象相关的关键化合物

• 批准号: 12045235
• 负责人: UEMURA Daisuke
• 金额: $ 18.56万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12045235/
• 关键词: Anesthetic substances; The short-tailed shrew Blarina brevicauda; The spider wasp Cyphononyx dorsalis; Isolation and structure determination; Causative agents of food poisoning; The Okinawan bivalve Pteria penguin; The Okinawan sponge Terpios hoshin

Nature has an enormous, almost incomprehensible, capacity for diversity and adaptation.We have investigated such challenging bioactive molecules to explore new frontiers and better understand basic concepts.We have sought to acquire a deeper understanding of biological phenomena.The American short-tailed shrew Blarina brevicauda is known to produce a potent venom.A novel lethal venom, blarina toxin, was isolated from the extract of its submaxillary gland.Mice injected with blarina toxin showed characteristic symptoms, i.e., irregular and quickened respiration, paralysis of the hind limbs, and convulsions before death.The mature form of the primary amino acid sequences of blarina toxin was determined, suggesting this protein was similar to tissue-kallikrein, a type of secretory serine protease.The spider wasp Cyphononyx dorsalis paralyze spiders as victims for their larvae with the stinging venom.Due to the interesting ecology of the wasp, we have studied these anesthetic substances gui … More ded by paralytic activity against spiders.Anesthetic substance (s) were separated using column chromatographies from the extract of the poison gland of the wasp.Amino acid sequences of the purified paralytic protein were analyzed by mass spectrometry.The protein was the new type of a kinase-like protein.The adductor muscle of the shellfish Pinna sp.is eaten in Japan and China, and food poisoning resulting from its ingestion occurs frequently.We successfully isolated pinnatoxins from the Okinawan bivalve P.muricata as a major cause of food, poisoning. Pinnatoxins are unique amphoteric carbocyclic compounds that possess novel Ca^<2+> channel-activating properties.We also isolated pteriatoxins A, B and C from the Okinawan bivalve Pteria penguin.We also focused on the marine sponge Terpios hoshinota, which overgrows coral reefs in waters off the Ryukya Islands.The ethanol extract of the sponge was separated using column chromatographies to give the new cytotoxic compounds, terpiodiene, nakiterpiosin and nakiterpiosinone.Nakiterpiosin, a novel C-nor-D-homosteroids, showed potent cytotoxicity. Less

大自然具有巨大的，几乎是不可理解的，多样性和适应能力。我们研究了这些具有挑战性的生物活性分子，以探索新的前沿领域，更好地理解基本概念。我们试图获得对生物现象的更深入的理解。美国短尾鼩 鼱 Blarina brevicauda已知产生一种强效毒液。一种新的致命毒液，Blarina毒素，从其颌下腺提取物中分离得到。注射布拉藻毒素的小鼠表现出特征性症状，即，呼吸不规则和加快，后肢麻痹，死亡前抽搐。Blarina毒素的初级氨基酸序列的成熟形式被确定，表明这种蛋白质类似于组织-激肽释放酶，一种分泌型丝氨酸蛋白酶。Cyphononyx dorsalis蜘蛛黄蜂用刺毒液麻痹蜘蛛作为其幼虫的受害者。由于黄蜂有趣的生态学，我们研究了这些麻醉物质 ...更多信息 本文报道了一种对蜘蛛具有麻痹活性的蛋白质。用柱层析法从该蜂毒腺提取物中分离出麻醉物质。用质谱法分析了纯化的麻痹蛋白质的氨基酸序列。该蛋白质是一种新型的类激酶蛋白质。日本和中国食用的贝类Pinna的闭壳sp.is肌我们从冲绳双壳类海产品中成功分离出了引起食物中毒的主要毒素--河豚毒素（pinnatoxins）。鳍状毒素是一种独特的两性碳环化合物，具有新型的Ca^2+通道激活特性。我们还从冲绳双壳类企鹅中分离出鳍状毒素A、B和C。我们还重点研究了生长在琉球群岛附近沃茨珊瑚礁上的海绵Terpios hoshinota。海绵的乙醇提取物用柱色谱分离得到新的细胞毒性化合物terpiodiene，Nakiterpiosin是一种新型的C-去甲-D-同型甾体，显示出强的细胞毒性。少

项目成果

K.Ito et al.: "Channel induction by palytoxin in yeast cells expressing Na^+,K^+-ATPase or its chimera with sarco/endoplasmic reticulum Ca^<2+>-ATPase"FEBS Letters. 543. 108-112 (2003)

K.Ito等人：“在表达Na+,K+-ATP酶或其与肌浆/内质网Ca+2-ATP酶的嵌合体的酵母细胞中通过海藻毒素进行通道诱导”FEBS快报。

H.Kigoshi et al.: "Enantioselective Synthesis of 15-epi-Haterumalide NA Methyl Ester and Revised Structure of Haterumalide NA"Organic Letters. 5. 957-960 (2003)

H.Kigoshi 等人：“15-epi-Haterumalide NA 甲酯的对映选择性合成和 Haterumalide NA 的修订结构”有机快报。

N.Takada et al.: "Structural determination of pteriatoxins A, B and C, extremely potent toxins from the bivalve Pteria penguin"Tetrahedron Letters. 42. 3495-3497 (2001)

N.Takada 等人：“来自双壳类企鹅 Pteria 的极强毒素 Pteriatoxins A、B 和 C 的结构测定”Tetrahedron Letters。

Y.Kan et al.: "Complete NMR signal assignment of palytoxin and N-acetylpalytoxin"Tetrahedron Letters. 42. 3197-3202 (2001)

Y.Kan 等人：“海鞘毒素和 N-乙酰海鞘毒素的完整 NMR 信号分配”四面体快报。

T.Teruya: "Nakiterpiosin and Nakiterpiosinone, Novel Cytotoxic C-nor-D-homosteroids from the Okinawan Sponge Terpios hoshinota"Tetrahedron. (in press). (2004)

T.Teruya：“Nakiterpiosin 和 Nakiterpiosinone，来自冲绳海绵 Terpios hoshinota 的新型细胞毒性 C-nor-D-同质类固醇”四面体。