Exchange of signal molecules derived from fungal parasites and plants on the plant surfaces and then-signal transmission leading to defense reaction or accessibility induction.

来自真菌寄生虫和植物的信号分子在植物表面进行交换，然后信号传输导致防御反应或可及性诱导。

• 批准号: 12052219
• 负责人: SHIRAISHI Tomonori
• 金额: $ 47.17万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12052219/
• 关键词: Qctadecanoic acid; AtMT method; Osmotin-resistant gene; Arabidopsis thaliana ecotype In-0; Extracellular matrix; Esterase; NTPase-transformd tobacco; Virus-inducing gene silencing; 根頭癌腫病菌による形質転換系(AtMT); 遺伝子ターゲテイング; ペルオキシゾーム; 細胞学マトリクス(ECM); 透過型

In this project, we found that, 1) A new inducer for germination of conidiospores of a fungal pathogen (Colktotrichum lagenarium) is a derivative of octadecanoic acid ; 2) A gene for resistance to osmotin-like proteins was cloned as s new virulent gene from C.lagenarium by AtMT method. Disruption of this ortholog in Magnaporthe grisea resulted in avirulent to rice plant ; 3) In-0, an ecotype of Arabidopsis thaliana showed resistance to C.higginsianum. This resistance gene is located the 4th chromosome with several molecular markers ; 4) Conidiospores of an incompatible powdery mildew fungus (Erysiphe pisi) secreted immediately extracellular matrix (ECM) including esterase on the surface of plants when these spores contacted. This ECM induced inaccessibility (resistance at cell level) to the compatible challenger ; 5) Cell wall-bound NTPase is a receptor for exogenous signal molecules. Inorganic phosphate, one of the products by NTPases induced defense response in plants. Transgenic tobacco with NTPase gene showed resistance to virulent fungi ; 6) VTGS of NTPase in Nicotiana benthamiana showed susceptibility to bacterial pathogens, indicating that NTPases are lightly associated with nonhost resistance.

本研究发现：1)一种新的真菌病原菌（Colktotrichum lagenarium）分孢子萌发诱导剂为十八烷酸衍生物；2)利用AtMT方法克隆了一个抗渗透蛋白样蛋白的毒力基因。该同源物在稻瘟病病菌中的破坏导致对水稻植株无毒；3)拟南芥生态型In-0对希金氏弧菌具有抗性。该抗性基因位于第4染色体上，有几个分子标记；4)一种不相容白粉病真菌（Erysiphe pisi）的分孢子孢子接触植物表面时，立即分泌包括酯酶在内的细胞外基质（ECM）。这种ECM诱导了对相容挑战者的不可接近性（细胞水平的抗性）；细胞壁结合的NTPase是外源信号分子的受体。无机磷酸盐是NTPases诱导植物防御反应的产物之一。NTPase基因转基因烟草对毒力真菌具有抗性；6)烟叶NTPase的VTGS表现出对细菌病原菌的易感性，表明NTPase与非寄主耐药有轻微关联。

项目成果

Gento Tsuji, Satoshi Fujii, Naoki Fujihara, Chika Hirose, et al.: "Agrobacterium tumefaciens-mediated transformation for random insertional mutagenesis in Colletotrichum lagenarium"Journal of General Plant Pathology. 69. 230-239 (2003)

Gento Tsuji、Satoshi Fujii、Naoki Fujihara、Chika Hirose 等人：“根癌农杆菌介导的炭疽菌随机插入诱变转化”普通植物病理学杂志。

Okamoto, S. et al.: "Inhibitory effect of aflastatin A on melanin biosynthesis by Colletotrichum laaenanum"Microbiology. 147. 2623-2628 (2001)

Okamoto, S. 等人：“黄黄他汀 A 对炭疽菌黑色素生物合成的抑制作用”微生物学。

Fujii,I.,Mori,Y.,Watanabe.A.,Kubo,Y. et al.: "Enzymatic synthesis of 1,3,6,8-tetrahydroxynaphthalene solely from malonyl coenzyme A by a fungal interactive typeI polyketide synthase PKS1."Biochemistry. 39. 8853-8858 (2000)

藤井 I.、森 Y.、渡边 A.、久保 Y.

Imura,Y.,Iguchi,S.,Toyoda,K.,Ichinose,Y.,Shiraishi,T.and Yamada,T.: "Importance of AC-rich element on pea phenylalanine ammonia-lyase gene 1 promoter for expression induced by nonpathogen attack."J.Gen.Plant Pathol.. 66. 123-127 (2000)

Imura，Y.，Iguchi，S.，Toyoda，K.，Ichinose，Y.，Shiraishi，T. 和 Yamada，T.：“富含 AC 元素对豌豆苯丙氨酸解氨酶基因 1 启动子诱导表达的重要性

Shiraishi,T. et al.: "Delivery and Perception of Pathogen Signals in Plants (N.Keen et al.eds.)"APS Press, St. Paul, Minnesota, USA. 268 (2001)

白石，T.