Mechanism in recognition of non-self sustained by the interaction between organelles in plant cell

植物细胞内细胞器间相互作用维持非自体的识别机制

• 批准号: 15108001
• 负责人: SHIRAISHI Tomonori
• 金额: $ 70.22万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (S)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15108001/
• 关键词: plant cell wall; apyrase (NTPase); signal transmission; cell cycle-related gene Cdc27B; ER-transnortation-related eene Rer1(6); defense reaction; MAMPs; WRKY; アピラーゼ(NTPase); サプレッサー; 宿主特異性; 植物免疫; 病害抵抗性植物; 非宿主抵抗性; アピラーゼ; 細胞壁; 植物; 免疫学; フラジェ

In this research, we attempted to clarify the plant defense mechanism in recognition of MAMPs, signal transmission and action of virulence effectors, mediated by the interaction between respective organelles. In this fiscal year, we showed that several molecules interacted with apyrase in plant cell wall by PAGE and TOF/MS/MS analyses. We also presented that MAMPs activated the cell-wall-bound apyrase, resulting in increasing inorganic phosphate that stimulated generation of active oxygen species and expression of defense-related genes. MAMPs also regulated the expression of NbCdc27B, which is a component of M phase in cell cycle. NbCdc27B participated in defense reaction without hypersensitive cell death Microarray analysis indicated that the transcriptional activation of WRKY41 was induced by MAMPs. Transformants highly expressed WRKY41 showed resistance to Pseudomonas syringae, but, inversely, susceptiblity to Erwinia carotovara, indicating that WRKY41 participated in SA signaling pathway. Transportation of proteins via endoplasmic reticulum may be also crucial to defense. Based on these findings, we discuss defense signaling sustained by respective organelles.

在本研究中，我们试图阐明植物在识别MAMPs、信号传递和毒力效应物的作用等方面的防御机制，这些机制是通过细胞器之间的相互作用介导的。在本财年，我们通过PAGE和TOF/MS/MS分析发现了几种分子与植物细胞壁中的apyrase相互作用。我们还发现，MAMPs激活细胞壁结合的apyrase，导致无机磷酸盐增加，从而刺激活性氧的产生和防御相关基因的表达。MAMPs还能调控细胞周期中M期组分NbCdc27B的表达。微阵列分析表明，WRKY41的转录激活是由MAMPs诱导的。高表达WRKY41的转化子对紫花假单胞菌表现出抗性，而对胡萝卜Erwinia则表现出易感性，表明WRKY41参与了SA信号通路。蛋白质通过内质网的运输也可能对防御至关重要。基于这些发现，我们讨论了各自细胞器维持的防御信号。

项目成果

イネにおけるOxidosqualene Cyclase遺伝子群のゲノム構造と遺伝子発現

水稻氧化角鲨烯环化酶基因组的基因组结构及基因表达

• 发表时间: 2007
• 作者: 稲垣善茂;睦門由果子;等
• 通讯作者: 等

Modulation of defense signal transduction by flagellin-induced WRKY41 transcription factor in Arabidopsis thaliana

• DOI: 10.1007/s00438-007-0315-0
• 发表时间: 2008-03-01
• 期刊: MOLECULAR GENETICS AND GENOMICS
• 影响因子: 3.1
• 作者: Higashi, Kuniaki;Ishiga, Yasuhiro;Ichinose, Yuki
• 通讯作者: Ichinose, Yuki

微生物の病原性と植物の防御応答

微生物致病性和植物防御反应

• 发表时间: 2007
• 作者: Murata;Y.;向原隆文;向原 隆文;向原 隆文
• 通讯作者: 向原 隆文

What is an assignment of physiological plant pathology today?-Opinion of an old phytopathologist walking at the wayside

今天生理植物病理学的任务是什么？-一位走在路边的老植物病理学家的意见

• 发表时间: 2007
• 期刊: In Dynamism of Plant-Pathogen Interactions (eds. by Y. Kubo, et. al.), Phytopathological Society of Japan (Tokyo)
• 作者: Shiraishi;T
• 通讯作者: T

今、感染生理学は何を明らかにすべきか?路傍から

感染生理学现在应该从路边揭示什么？

• 发表时间: 2007
• 作者: Tetsuo Katsuura;Jinghua Huang;Xinqin Jin;Xinxin Liu;Yoshihiro Shimomura;Koichi Iwanaga;白石友紀
• 通讯作者: 白石友紀