Analysis of 14-3-3 function and integration in signaling networks of Arabidopsis

拟南芥14-3-3功能及信号网络整合分析

• 批准号: 49886009
• 负责人: Professorin Dr. Claudia Oecking
• 金额: --
• 依托单位: Abteilung für Pflanzenphysiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/49886009?language=en
• 关键词: Analysis; 14; function; integration; signaling

Members of the eukaryotic 14-3-3 family are highly conserved proteins that have been implicated in the regulation of distinct biological processes and pathways by protein-protein interactions. Most notably, 14-3-3 proteins bind to phosphoserine/phosphothreonine motifs in a sequence-specific manner and are required to induce a change in the activity state of the respective target protein. Therefore, 14-3-3s are involved in co-ordination and cross-talk of diverse signaling processes. Arabidopsis harbors thirteen 14-3-3 isoforms, most of the functions of which, in particular with regard to the signaling pathways in which they participate, are currently unknown. Since 14-3-3s physically interact with other polypeptides, a comprehensive analysis of their function will provide insights into functional networks of protein families in plants. Therefore, the proposed project aims at identifying proteins that interact with selected 14-3-3 isoforms. In addition, we will attempt to characterize specific physiological processes regulated by different 14-3-3 isoforms or isoform subgroups. Taken together, these studies are expected to broaden our understanding of 14-3-3 function and integration in signaling networks of Arabidopsis.

真核14-3-3家族的成员是高度保守的蛋白质，通过蛋白质-蛋白质相互作用参与了不同生物过程和途径的调节。最值得注意的是，14-3-3蛋白以序列特异性的方式与磷丝氨酸/磷苏氨酸基序结合，并且需要诱导各自靶蛋白活性状态的变化。因此，14-3-3参与多种信号过程的协调和串扰。拟南芥含有13种14-3-3亚型，其中大部分功能，特别是它们参与的信号通路，目前尚不清楚。由于14-3-3 -3与其他多肽具有物理相互作用，因此对其功能的全面分析将有助于了解植物蛋白质家族的功能网络。因此，提出的项目旨在鉴定与选定的14-3-3亚型相互作用的蛋白质。此外，我们将尝试表征不同14-3-3异构体或异构体亚群调节的特定生理过程。综上所述，这些研究有望拓宽我们对拟南芥14-3-3功能和信号网络整合的理解。