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Pax6変異ラット脳に見られるアストロサイトの異常と神経機能異常との関連

Pax6突变大鼠脑中星形胶质细胞异常与神经功能障碍的关系

基本信息

批准号：
16047202
负责人：
野村真
金额：
$ 4.74万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

我々はこれまでに、転写因子をコードするPax6遺伝子の変異ラットでは海馬の神経新生が低下しており、またアストロサイトの形態異常が認められることを見いだしている(Maekawa et al.,2005)。一方、行動学的解析により、Pax6変異ラットでは社会性の異常などが認められており、このラットは生後神経新生やアストロサイトの形態と脳高次機能との関連を解析する上での格好のモデル動物である。そこで本年度は、生後神経新生におけるPax6の機能を詳細に解析するため、Pax6の下流制御遺伝子としてのfabp7(fatty acid binding protein)に着目し、FABP7発現細胞の性質を検討した。その結果、野生型ラット海馬では、FABP7発現細胞のほとんどがPax6を共発現しており、またPax6変異ラットではFABP7発現細胞数が減少していた。胎生期脳ではFABP7は神経上皮細胞の未分化性維持に機能していることから(Arai et al. 2005)、生後海馬においてもFABP7はPax6の標的遺伝子として神経新生の制御に関わっている可能性が考えられる。またPax6は生後大脳皮質のGFAP陽性アストロサイトにも発現が見られる。In vivo、in vitroにおいて大脳皮質アストロサイトの形態を検討した結果、野生型と比べてPax6変異ラットのアストロサイトは突起伸展率が低下していた。以上の結果から、転写因子Pax6はFABP7を介して神経幹細胞の増殖を制御し、また分化したアストログリアの形態を制御している可能性が示唆された。

I would like to know that the factors are not correct, that is, the factors are Pax6, the child is the child, the sea is born, the god is born, the baby is low, the shape is often the same, and the shape is always the same (Maekawa et al.,2005). On the one hand, the analysis of the dynamics, the Pax6, the social, the, the, In this year's and post-natal life, the Pax6 machine was able to analyze the virus, the Pax6 system was used to make the device fabp7 (fatty acid binding protein), and the FABP7 was shown to be sensitive. The results showed that the number of cells in the wild type was significantly lower than that in the wild type, and the number of cells in the wild type was significantly lower than that in the FABP7. The results showed that the number of cells in the wild type was significantly lower than that in the wild type, and the number of cells in the wild type was significantly lower than that in the FABP7. The results showed that the number of cells in the wild type was significantly lower than that in the wild type, and the number of cells in the wild type was significantly lower than that in the wild type. The results showed that the number of cells in the wild type was significantly lower than that in the wild type, and the number of cells in the wild type was significantly lower than that in the FABP7. The results showed that the number of cells in the wild type was significantly lower than that in the wild type, and the number of cells in the wild type was significantly lower than that in the wild During the prenatal period, the undifferentiated nature of the epithelial cells of the fetal FABP7 cells was maintained and the mechanism was used to express the undifferentiated cells (Arai et al). 2005), in the afterlife of the sea, the son of the FABP7 Pax6, the son of the god, the birth of the god, the possibility of the birth of the baby, the possibility of the birth of the child, the possibility of the birth of the child. After the birth of Pax6, the skin was GFAP and the sex was abnormal. The results of In vivo and in vitro test showed that the outgrowth rate of the wild type was lower than that of the wild type, and that of the wild type was lower than that of the Pax6. The above results show that the factors Pax6, FABP7, and so on indicate the possibility of cell proliferation control, differentiation, and morphological control.