High resolution X-ray crystal structural analysis of biological macromolecular assemblies

点击翻译按钮获取中文摘要

The goal of this research was to establish strategies by which we overcame limitation of molecular size and resolution in crystal structure analysis of biological macromolecular assemblies. We had two research plans. The first was to locate hydrogen atoms of cytochrome c oxidase with a molecular weight of 420 KDa at 1.6 A resolution. The second was to determine the structure of vault with a molecular weight of 10 MDa.Improving diffraction experiment method at BL44XU of SPring-8, we have successfully collected diffraction data of cytochrome c oxidase at 1.6 A resolution. We confirmed that the data set contained information of hydrogen atom positions. Structures of connexin26 gap junction channel and Exportin-5:RanGTP:pre-miRNA complex were determined by using intensity data sets acquired at the BL44XU.The structure of vault was determined by applying a newly developed method.