High resolution X-ray crystal structural analysis of biological macromolecular assemblies

生物大分子组装体的高分辨率X射线晶体结构分析

• 批准号: 16087206
• 负责人: TSUKIHARA Tomitake
• 金额: $ 125.63万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16087206/
• 关键词: 生体超分子; 構造; 機能; X線結晶構造解析; X 線結晶構造解析

The goal of this research was to establish strategies by which we overcame limitation of molecular size and resolution in crystal structure analysis of biological macromolecular assemblies. We had two research plans. The first was to locate hydrogen atoms of cytochrome c oxidase with a molecular weight of 420 KDa at 1.6 A resolution. The second was to determine the structure of vault with a molecular weight of 10 MDa.Improving diffraction experiment method at BL44XU of SPring-8, we have successfully collected diffraction data of cytochrome c oxidase at 1.6 A resolution. We confirmed that the data set contained information of hydrogen atom positions. Structures of connexin26 gap junction channel and Exportin-5:RanGTP:pre-miRNA complex were determined by using intensity data sets acquired at the BL44XU.The structure of vault was determined by applying a newly developed method.

本研究的目的是建立克服生物大分子组装体晶体结构分析中分子尺寸和分辨率限制的策略。我们有两个研究计划。第一个是在1.6 A分辨率下定位分子量为420 KDa的细胞色素c氧化酶的氢原子。第二部分是对分子量为10 MDa的细胞色素c氧化酶穹窿结构的测定，改进了在SPring-8的BL 44 XU上的衍射实验方法，成功地获得了1.6A分辨率的细胞色素c氧化酶的衍射数据。我们证实了数据集包含氢原子位置的信息。利用BL 44 XU获得的强度数据集确定连接蛋白26间隙连接通道和Exportin-5：RanGTP：pre-miRNA复合物的结构，并应用新开发的方法确定穹窿结构。

项目成果

Structure of coenzyme A-disulfide reductase from Staphylococcus aureus at 1.54 Å resolution

• DOI: 10.1021/bi061139a
• 发表时间: 2006-09-26
• 期刊: BIOCHEMISTRY
• 影响因子: 2.9
• 作者: Mallett, T. Conn;Wallen, Jamie R.;Claiborne, Al
• 通讯作者: Claiborne, Al

Rotational symmetry of vault from rat liver

大鼠肝脏穹窿的旋转对称性

• 发表时间: 2007
• 作者: Wada Y;Tanaka H;Yamashita E;Ichiki-Uehara T;Omura T and Tsukihara T;田中秀明;加藤公児;和田康史;加藤 公児;田中 秀明
• 通讯作者: 田中 秀明

Absolute configuration of the hydroxyfarnesylethyl group of haem A, determined by X-ray structural analysis of bovine heart cytochrome c oxidase using methods applicable at 2.8A resolution

血红素 A 的羟基法呢基乙基的绝对构型，通过牛心细胞色素 C 氧化酶的 X 射线结构分析，使用适用于 2.8A 分辨率的方法来确定

• 发表时间: 2005
• 期刊: Acta Crystallographica section D D61
• 作者: Yamashita E.;Aoyama H.;Yao M.;Muramoto K.;Shinzawa-Itoh K.;Yoshikawa S.;Tsukihara T.
• 通讯作者: Tsukihara T.

Preliminary crystallographic analysis of the antibiotic discharge outer membrane lipoprotein OprM of Pseudomonas aeruginosa with an exceptionally long unit cell and complex lattice structure

超长晶胞和复杂晶格结构的铜绿假单胞菌抗生素排出外膜脂蛋白OprM的初步晶体学分析

• 发表时间: 2005
• 期刊: Acta Cryst. F61
• 作者: Akama;H.
• 通讯作者: H.

Structure and Function of Cytochrome c Oxidase, The 19th Symposium on Role of Metals in Biological Reactions

细胞色素c氧化酶的结构与功能，第十九届金属在生物反应中的作用研讨会

• 发表时间: 2009
• 作者: 清水巧;Taiyun Wei;宮崎直幸;一木(植原)珠樹;日比野啓行;岩崎憲治;大村敏博;月原冨武
• 通讯作者: 月原冨武