Crystal Structural Analysis of IL6/IL6 receptor Complex and Development of Functional Regulation of IL6 with cDNA

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1. Atotal of 150 mg AUK18l 6 was purified from 30 ml of abdominal dropsy. Sil6 receptor affinity column was prepared by using AUK181 6. 6 mg of Sil6 receptor was purified from CHO cells producing Sil6 receptor.2. Structure of IL 6 in the novel crystal form was determined at 2.3 A resolution. The crystal shucture showed a loop structure that is not visible in the crystal structure previously determined. This loop interacts probably with IL6 receptor.3. Inhibition of IL6 by a FELRY peptide that bind to IL6 was inspected. The FELRY at 0.2 to 25 % saturation was added to IL6 dependent cells under rIL6 stimulation. Significant suppression of cell growth was not detected. Other soluble peptides are in under inspection.4. The method for molecular observation by AFM was developed to analyze recognition among IL6, IL6 receptor and gp130.