X-ray crystallographic studies of intr- and inter-cellular transport

进行了三种与交通有关的结构研究。第一个是pre-miRNA的核输出机制。第二部分是承载单元中材料的拱顶结构研究。第三部分是缝隙连接通道的结构-功能研究，分别在2.9、3.0和3.5 A分辨率下测定了Exportin-5：RanGTP：Pre-miRNA复合物、Exportin-5：RanGTP复合物和Exportin-5的结构。比较Exportin-5在3种不同状态下的结构，分析Exportin-5与Pre-miRNA的相互作用，阐明Exportin-5：RanGTP复合物转运Pre-miRNA的机制。我们已经确定了39倍对称的MVP-拱顶除了拱顶从大鼠肝脏。因此，拱顶的39重对称性得到了证实。我们阐明对接选择性规则的半通道对同源建模的基础上连接蛋白26间隙连接通道和功能分析。

Three kinds of structural research relating to the transportation were carried out. The first is the nuclear export mechanism of pre-miRNA. The second is structural study of vault that carries materials in the cell. The third is structural-functional studies of gap junction channels.Structures of Exportin-5:RanGTP:Pre-miRNA complex, Exportin-5:RanGTP complex and Exportin-5 were determined at respective 2.9, 3.0 and 3.5 A resolutions. Comparing structures of exportin-5s in three different states and analyzing interactions between Pre-miRNA and Exportin-5, we elucidated Pre-miRNA transportation mechanism by Exportin-5:RanGTP complex. We have determined the 39-fold symmetry for the MVP-vault in addition to the vault from rat liver. Consequently, the 39-fold symmetry of vault was confirmed. We elucidated docking selectivity rules for hemichannel pairs by homology modeling based on the connexin-26 gap junction channel and functional analyses.