Characterization of roles of PRL-CDC5 complex in regulation of pre-mRNA splicing and flowering time

真核生物剪接体的组装和激活需要CDC5 Myb因子与prl型WD-40蛋白的相互作用。我们的数据表明，prll和cdc5突变在包括开花时间控制在内的多种发育和信号通路的调控中引起类似的同时变化。本研究旨在研究PRL和cdc5突变引起的pre-mRNA剪接缺陷、转录控制和开花时间和花朵发育调控改变之间的因果关系，以及PRL/ cdc5交联的pre-mRNA和cdc5 myb结合的染色质靶点的鉴定。

Interaction of CDC5 Myb factors with PRL-type WD-40 proteins is required for Spliceosome assembly and activation in eukaryotes. Our data show that prll and cdc5 mutations cause similar simultaneous changes in the regulation of multiple developmental and signaling pathways including the control of flowering time. The proposed studies aim at the characterization of causal relationships between defects in pre-mRNA splicing, transcription control and alterations in the regulation of flowering time and flower development caused by the prll and cdc5 mutations, as well as the identification of PRL/CDC5 cross-linked pre-mRNA and CDC5 Myb-bound chromatin targets.